中文摘要：

本试验旨在研究高盐诱发肉鸡腹水综合征（AS）过程中肺小动脉壁癌基因c-jun mRNA表达的规律，初步探讨肺小动脉壁癌基因c-jun mRNA表达在AS发生过程中的可能作用。将100只1日龄商品代AA雄性肉仔鸡常规育雏，8日龄起随机分为对照组和高盐组（饮水中添加0．30％氯化钠）。分别在15、22、29、36、43、50日龄时每组随机取6只鸡的肺组织制备切片，以原位杂交法结合图像分析法测定肺小动脉壁癌基因c-jun mRNA的表达。结果表明：对照组肉鸡生长发育过程中肺小动脉壁癌基因c-jun mRNA表达呈现动态变化，22日龄是其表达高峰。15、29、36日龄时高盐组各级肺小动脉壁c-jun mRNA相对含量极显著高于对照组（P〈0．01）。43、50日龄时高盐组仅外径20～50μm肺小动脉壁c-jun mRNA相对含量极显著高于对照组（P〈0．01）。提示高盐促进了肉鸡肺小动脉壁癌基因c-jun mRNA表达，尤其是在外径20～50μm的肺小动脉壁。肺小动脉壁癌基因c-jun mRNA的过度表达可能参与了高盐诱发AS的过程。

英文摘要：

In this study, the expression of c-jun mRNA in the wall of pulmonary arterioles was investigated to clarify its possible effect during the development of AS induced by excess salt in broiler chickens. One hundred 8 days old male Arbor Acres broilers were randomly divided into control group（C） and treated group （T）. Birds in the treated group were supplied with drinking water added 0.30% NaCl, and other factors were same as that in control group. Lung samples of 6 randomly selected birds per group were collected at 15, 22,29,36,43 and 50 days old respectively to make routine paraffin section. Expression of c-jun mRNA was determined by in situ hybridization（ISH） and image analysis program. The results showed that there was dynamic expression of c-jun mRNA in the wall of pulmonary arterioles of broiler chickens, c-jun mRNA was expressed mostly at 22 days old in the control group. The relative content of c-jun mRNA in the wall of all pulmonary arterioles in the treated group was significantly higher than that of control group at 15, 29 and 36 days old （P〈0.01）, but at 43 and 50 days old significant difference were found only in outer diameter 20-50 μm pulmonary arterioles wall （P〈0. 01）. These results suggested that excess salt accelerated expression of c-jun mRNA particularly in outer diameter 20- 50 μm pulmonary arterioles wall,and that expression of c-jun mRNA may play an important role during the development of AS.