中文摘要：

目的：观察骨骼肌损伤修复过程中肌再生调节因子和血管再生因子表达规律，探讨肌再生调节因子和血管再生因子在骨骼肌损伤修复过程中可能发挥的作用。方法：40只雄性C57小鼠随机分为对照组（C组，11=8）和损伤组（S组，n=32）。骨骼肌钝挫伤后0d，1d，3d，7d，14d取右侧腓肠肌用于HE染色，左侧腓肠肌进行荧光定量PCR检测肌再生调节因子（MyoD，myogenin，IGF-1，MGF,HGF,uPA，Myostain，GDF11，LIF）和血管再生因子（HIF—1α，Angpt-1，VEGF）mRNA表达变化。结果：①骨骼肌钝挫伤后第3天有少量再生肌纤维，第7天显著增加，第14天基本恢复正常。②与对照组相比，生肌调节因子（MyoD，myogenin，IGF-1，MGF，HGF,uPA，GDF11，LIF）mRNA在损伤后表达显著增加（P〈0．05或P〈0．01），MyostainmRNA在损伤后表达显著降低（P〈0．01）。③血管再生因子（HIF—1α，Angpt1）mRNA在损伤后表达显著增加（P〈0．05或P〈0．01）而VEGFmRNA在损伤后表达显著降低（P〈0．05）。结论：骨骼肌钝挫伤后多种肌再生调节因子表达上调，可能促进骨骼肌再生。血管再生因子（HIF—1α和Angpt1）表达上调，可能参与了骨骼肌损伤后血管再生以及骨骼肌再生。

英文摘要：

Objective： To confirm the roles of muscle regeneration regulatory factors and angiogenesis factors over skeletal muscle injury and repair process by investigating the changes of muscle regeneration regulatory factors and angiogenesis factors after injury.Method： Forty C57 male mice were divided into control group （C, n=8） and muscle contusion group （S, n= 32）. Subsequently, their gastrocnemius muscles were harvested at 0d, 1d, 3d, 7d, 14d after injury. HE stain were used to assess the changes of muscle morphology. In addition, the gene expression of regeneration regulatory factors and angiogenesis factors was analyzed by real-time polymerase chain reaction.Result： ①Morphology of skeletal muscles showed signs of regeneration at 3d post injury. The maximum amount of regeneration muscle fibers appeared at one week post contusion. Two weeks post-injury morphology of myofibers nearly recovered to normal. ②After injury, compared with group C, the regulatory factors for muscle regeneration （MyoD, myogenin, IGF-1, MGF, HGF, uPA, GDFll, LIF） mRNA increased significantly （P 〈 0.05 or P 〈 0.01）, the Myostain mRNA reduced significantly （P 〈 0.01）. ③The data showed that the expression of angiogenesis factors （HIF-1α, Angptl） mRNA increased significantly after muscle contusion （P〈0.05 or P〈 0.01）, while VEGF mRNA significant reduced post injury （P〈 0.05）. Conclusion： The expression regulatory factors for muscle regeneration up regulated after muscle contusion, may play important role in skeletal muscle regeneration. Angiogenesis factors up regulated also after muscle contusion, it may involve in muscle angiogenesis and regeneration.