中文摘要：

通过甲基丙烯酰氯和二甘醇胺反应设计合成了一种交联剂（2-甲基丙烯酰胺）乙氧基-2-甲基丙烯酸乙二醇单酯，以自行合成的（2-甲基丙烯酰胺）乙氧基-2-甲基丙烯酸乙二醇单酯为交联剂，丙烯酰胺为功能单体，合成了-种用于手性分离的N-叔丁氧羰酰-L-色氨酸（N-Boc-L-Trp，N-[（tert-butoxy）carbonyl]-tryptophan）的分子印迹聚合物。评价了该分子印迹聚合物作为高效液相色谱柱填料对Ⅳ-叔丁氧羰酰-色氨酸（N-Boc-Trp）的手性拆分能力。结果表明：当检测波长为280nm，流动相为V（甲醇）：V（水）=80：20，流速0．8mL/min时，该填料实现了对N-Boc-Trp外消旋混合物的手性拆分，显示出良好的选择识别性能。

英文摘要：

A new cross-linking monomer 2-（methacryloylamino）ethyoxyl -2-methacrylate ethandiol ester was designed, synthesized by methacryloyl chloride and 2-（2-aminoethoxy） ethanol. A kind of molecular imprinting polymers （MIPs） of N-[ （tert-buloxy） carbonyl ]-L-tryptophan （N-Boc-L-Trp） for chiral separation were synthesized using the 2-（methacryloylamino） ethyoxyl-2-methacrylate ethandiol ester as cross-linking monomer and acrylamide as function monomer. The chiral separation capability of this MIPs to N-BOC-tryptophan （N-Boc-Trp） was evaluated when it worked as a HPLC column filling. The experimental results showed that the chiral separation of N-Boc-Trp racemic mixture was performed by this MIPs and shown an excellent selective distinguish ability when detector wavelength is 280nm, mobile phase is methanol/water （80：20, v/v） and flow rate is 0.8 mL/min.