中文摘要：

实验小鼠具有体型小，基因特性明确，转基因或突变体品系多，饲养方便，繁殖迅速等优点，已被广泛应用于现代生物学、医学、药理学和心理学研究。在大量采用小鼠为受试对象的同时，国内外研究者也越来越重视实验动物的保护。最大限度地利用实验动物，并尽可能地减少动物使用量，这也是国内外实验室一直追求并建立的理念。本文总结了本实验室快速分离获取小鼠不同脑区新鲜组织用于蛋白免疫印迹和神经细胞培养的经验，旨在为实验初学者提供指导的同时，也为提高实验动物的利用率和降低动物使用数量提供思路和方法。

英文摘要：

Laboratory mice are common experimental animals in biological, medical, pharmacological and psychological researches primarily because they are easy to maintain and reproduce quickly. The protection of the welfare of experimental animals is gaining greater attention during the application of a large number of mice. It＇s therefore essential to consider how to reduce the unnecessary use of animals and fully exploit each experimental animal. We report, in this article, an efficient way to dissect various brain regions from a mouse for protein immunoblot and/or neuronal culture, providing technical reference information for minimizing the number of animals used in projects, and refining methods and procedures to quick brain dissection.