中文摘要：

目的采用RNA干扰与噬菌体肽库展示技术2种方法干预宫颈癌HeLa细胞CD59的表达，以探讨比较2种CD59低表达方式对HeLa细胞增殖与凋亡的影响。方法将10μg／mL的CD59短肽封条作用于HeLa细胞8h后，联合CD59干扰质粒pSUPER-siCD59稳定转染HeLa细胞系，采用MTr法检测各组细胞增殖率的变化，采用TUNEL染色、annexinV—PE／7-AAD染色结合流式细胞术检测细胞凋亡。结果CD59短肽封条组与pSUPER-siCD59质粒稳定转染细胞组HeLa细胞增殖明显弱于对照组，TUNEL及流式细胞术检测结果显示2组处理细胞均存在细胞凋亡现象，且短肽封条的效果要好于pSUPER-siCD59质粒。结论下调CD59表达能抑制其细胞增殖并促进其凋亡，且短肽封条效果要优于CD59RNA干扰质粒。

英文摘要：

Objective To intervene the expression of CD59 on cervical cancer HeLa cells by RNA interference and phage display random peptide library, respectively, and detect the effect of down-regulated CD59 expression on proliferation and apoptosis of the cervical cancer cells. Methods HeLa cells were divided into normal control group, CD59 peptide seal group, pSUPER transfected group and pSUPER-siCD59 transfected group. The CD59 peptide seal group was treated with 10 μg/mL CD59 peptide seal for 8 hours. The pSUPER and pSUPER-siCD59 transfected groups were transfected with empty plasmid pSUPER and recombinant plasmid pSUPER-siCD59, respectively. MIF assay was used for the detection of cell proliferation. TUNEL, annexin V-PE/7-AAD staining combined with flow cytometry were adopted for the detection of cell apoptosis. Results Compared with the control groups, both CD59 peptide seal group and pSUPER-siCD59 transfected group presented a diminished cell proliferation activity and a strengthened apoptosis. What＇s more, the effect of peptide seal on CD59 expression inhibition was better than that of pSUPER-siCD59 plasmid. Conclusion Down-regulation of CD59 expression could inhibit HeLa cells＇ proliferation and promote apoptosis, and the inhibitory effect of peptide seal was better than that of CD59 interference plasmid.