利用微生物电池对微生物在矿物表面电子传递的过程进行了实验研究.结果表明:Geobacter metallireducens还原Fe(OH)3过程中直接接触方式起着重要作用,而微生物在矿物表面吸附形成的生物膜是一个关键因素,生物膜的形成又是一个相对较长的过程;细胞在固体表面的吸附并成膜是一种重要的代谢途径,而电子传递中间体AQDS虽然能在初期有效加快还原速率,但是当细胞吸附完成后,其作用就不再显著了,说明微生物催化矿物氧化还原反应动力学受生物膜控制.加速微生物在矿物表面成膜及保持其稳定性是影响微生物浸矿速率的重要因素.
The electron transfer process of microbe on the mineral surface was studied by the use of a microbe fuel cell. The results indicate that direct contact plays an important role in the process of reducing Fe(OH)3 with Geobacte metallireducens, and the bio-film absorbed on the mineral surface is a key factor. Bio-film formation needs a relative long time. It is an important metabolic way to form bio-film by cell-absorption on the solid surface. Although mediate AQDS accelerates the reduction rate effectively at the early stage, but its effectiveness is not significant after the cell-absorption. So the microbe catalysis mineral oxidation-reduction reaction is influenced by bio-film. Accelerating the film formation on the mineral surface and keeping its stability are very important for the microbe leaching rate.