中文摘要：

[目的]探讨多药耐药相关基因如缺氧诱导因子1d（HIF-1α）、多药耐药基因（MDR1）及多药耐药相关蛋白（MRP1）在脊索瘤细胞系CM－319中的表达及临床意义。[方法]用RT—PCR检测HIF－1α、MDR1、MRP1基因表达，间接免疫荧光及免疫细胞化学Envision法检测HIF－1α、MRPl蛋白在CM－319中的表达情况，并分析其与放化疗抵抗的关系。[结果]常氧条件下，在脊索瘤细胞系中能检测到HIF～1α及MRP1 mRNA的表达，但未检测到MDR1 mRNA的表达（P〈0．01），间接免疫荧光及免疫细胞化学可以检测到HIF-1α表达在细胞浆及核中，而MRP1主要表达在细胞浆中。[结论]HIF-1α与MRP1的过度表达在脊索瘤的放化疗抵抗中可能具有重要作用，而MDR1表达未被检测到，提示脊索瘤的放化疗抵抗主要与HIF－1α和MRP1相关。

英文摘要：

[ Objective ] To investigate the expression of some genes related to resistance to chemotherapy and radiotherapy, such as HIF- 1α, MRP1 and MDR1 , and their clinical significance in chordoma cell line CM -319. [ Methods] The expression of HIF - 1α, MDR1 and MRP1 was detected with reverse transcription polymerase chain reaction （ RT - PCR） and indirect immunofluorescence staining and immunocytochemical technique （ChemMateTM Envision）. The relationship between HIF -1α, MRP1 and resistance to chemotherapy and radiotherapy was statistically analyzed. [ Results ] The positive expression of HIF-1α and MRP1 was detected in CM -319 in normal Oxygen conditions, while MDR1 was not detected by RT- PCR（P 〈0.01 ）. The positive expression of HIF- 1α protein was detected in cytoplasm and nucleus, and MRP1 mostly in cytoplasm with indirect immunofluorescenee staining and immunocytochemieal technique. [ Conclusions ] The overexpression of HIF - 1α, MRP1 plays an important role in the resistance to chemotherapy and radiotherapy of chordoma cell line. And the expression of MDR1 was not detected. It may be a signature that the resistance to chemotherapy and radiotherapy was related with HIF - 1α, MRP1 in chordoma cell line.