中文摘要：

目的：建立反相离子对色谱法同时测定左金丸中吴茱萸碱、吴茱萸次碱、盐酸药根碱、硫酸表小檗碱、硫酸黄连碱、盐酸巴马汀和盐酸小檗碱7个生物碱的含量。方法：以Diamonsil C18（4．6mm×250mm，5μm）为色谱柱；流动相A为1．5mmol·L^-1十二烷基硫酸钠溶液（以磷酸调pH5．0），流动相B为乙腈，梯度洗脱[0min，A—B（65：35）；10min，A—B（50：50）；16min，A—B（25：75）]；检测波长：265nm；流速：0．8mL·min^-1；柱温：25℃。结果：在30min内可完成对左金丸中7个生物碱的分离测定，各生物碱的进样量与峰面积线性关系良好（r=0．9985～0．9998）；加样回收率为94．5％-104．7％；信噪比为3时，检出限为0．11—0．47μg·mL^-1。结论：该法准确、稳定，重复性好，可用于左金丸的质量控制，也为含黄连、吴茱萸的成方制剂的质量控制提供了参考。

英文摘要：

Objective ：To establish a reversed phase - ion pair chromatography method for simultaneous determination of seven alkaloids including evodiamine, rutacarpine, jateorrhizine hydrochloride, epiberberine sulfate, coptisine sulfate,palmatine hydrochioride and berberine hydrochloride in Zuojin pills. Method： Simuhaneous separation of seven alkaloids was achieved on Diamonsil C18 column （4.6 mm × 250 mm,5 μm）. Under the condition of gradient elution, seven alkaloids were separated within 30 minutes. The mobile phase A was 1.5 mmol · L^-1 sodium dodecyl sulfate （ adjusted pH to 5.0 with phosphoric acid）, the mobile phase B was acetonitrile. The ratios of the contents of mobile A and B were 65：35 at 0 min,50：50 at 10 rain,25：75 at 16 min. The flow rate was 0. 8 mL · min^-1 ,the column temperature was 25 ℃. Results：All calibration curves showed good linear regression within test ranges（r = 0. 9985 - 0. 9998 ）. The recoveries ranged from 94.5% to 104. 7%. The limit of detection （LOD） ranged from 0. 11 to 0. 47 μg·mL^-1（ S/N= 3 ） for seven alkaloids. Conclusion： This method is simple and accurate, can be used for the simultaneous determination of seven alkaloids in Zuojin pills, and it also provides reference for quality control of Chinese medicine preparations, including Rhizoma Coptidis and Fructus Evodiae.