中文摘要：

中药提取物是传统中药融合现代制药技术的新型产品,具有广泛的国际市场。建立快速、有效的分析方法有助于中药提取物的实时质量监控。文章利用傅里叶变换红外光谱技术（FT-IR）对黄连药材、水提物和醇提物作了研究。结果表明：黄连药材及其提取物有着各自稳定的光谱特征,谱图中位于1505 cm^-1的芳环骨架振动峰是判断盐酸小檗碱在不同黄连样品中含量高低的主要依据,而相应的二阶导数谱图可以大大提高原始谱图的分辨率。黄连药材经过提取,以小檗碱为代表的主要活性成分得到有效富集,两种提取物中小檗碱的含量明显增高;黄连醇提物中小檗碱的含量又明显高于其水提物。FTIR能够简便、快速地提供中药提取物中主要化学成分的定性与半定量信息,从而为后续的化学成分分析和中药提取分离工艺的改进提供有效参考。

英文摘要：

In the present paper,we applied Fourier transform infrared spectroscopy（FTIR） and second derivative infrared spectroscopy to establish a rapid and efficient analytical method for a preliminary quality control of medicinal herb extracts.The commonly used Chinese herb medicine,Rhizoma coptidis,was taken as an example.The main component-berberine in rhizoma coptidis was analyzed as target compound.The results indicated that the IR spectra of rhizoma coptidis herb,its water and alcohol extracts,exhibit their macroscopic fingerprint characters with fine repeatability,and the corresponding secondary derivative spectra further confirmed the spectral characteristics of the original IR spectra by enhancing spectral resolution.The typical and strongest absorption band of IR spectrum of berberine is at 1 505 cm-1,which is assigned to aromatic skeleton vibration band,and its intensities in spectra of rhizoma coptidis herb and its different extracts become the main identification standard of the contents of berberine in those samples.In addition,comparing the IR spectra of the medicinal herb and its extracts,it was shown that the main bioactive components,alkaloids in rhizoma coptidis herb,are enriched by extraction,and the contents of berberine in alcohol extracts of rhizoma coptidis are higher than those in water extracts.The spectroscopic technique described is a simple and rapid analytical technique,which could provide valuable information about chemical constituents of medicinal extracts for guiding further chromatographic analysis and separation improvement.