中文摘要：

摘要目的：以典型的迟发型超敏反应模型小鼠变应性接触性皮炎及脾淋巴细胞IFN-γ、IL-4的分泌，筛选玉屏风散抑制迟发型超敏反应的效应部分。方法：用二硝基氯苯来诱导小鼠变应性接触性皮炎，观察玉屏风散不同分离部分的干预作用；用MTF法检测玉屏风散不同分离部分对小鼠脾脏T淋巴细胞活化增殖的影响；采用ELISA法检测IFN-γ、IL-4的水平。结果：玉屏风散的醇提浓缩液能减轻小鼠耳廓的肿胀程度、显著抑制ConA诱导的T淋巴细胞增殖及IFN-γ、IL-4的分泌；乙酸乙酯萃取物能减轻小鼠耳廓的肿胀程度，显著抑制C0nA诱导的IFN-γ分泌，促进IL-4分泌；石油醚萃取物能抑制ConA诱导的IFN-γ、IL-4的分泌，整体模型未显示作用。三种提取物都不同程度地降低IFN-γ／IL-4比值，抑制Th1反应。结论：玉屏风散中抗迟发型超敏反应的效应成分主要在醇提液和乙酸乙酯部分，其作用与调节Th1／Th2的平衡有关；同时也提示以散剂为应用剂型是合理的。

英文摘要：

AIM： To screen immunoregulatory components by evaluating the effects of different extracts of Yu-ping-feng powder on allergic contact dermatitis （ACD） which is a well known animal model for delayed type hypersensitivity（DTH）. Proliferation of T lymphocytes and secretion of the cytokines induced by ConA were also applied to evaluate the effects of extracts of Yu-ping-feng powder. METHODS： Mice ACD were induced by DNCB and the proliferation of T lyanphocytes was measured by MTT. The levels of cytokines were determined by ELISA. RESULTS： The swelling degree of mice auricle was reduced significantly by the alcohol extract substance. The T cell proliferation and IFN-γ/IL-4 secretion stimulated by ConA were inhibited significantly by this site. Significant effects were also found in the acetic ether extract parts treated groups including reduction of DTH in vivo, inhibition of IFN-γ/production and increase of IL-4 level in vitro. The ligarine extract part was found to inhibit IFN-γ and IL-4 secretion in vitro while no significant effect was detected in mice model. As far as the IFN-γ/IL-4 ratio was concerned, the alcohol extract part, the ligarine extracting site and the acetic ether extract- ing site had certain inhibitory action. CONCLUSION： The active components for DTH are wherein the alcohol extracting site and the acetic ether extracting site of Yu-ping-feng powder and their effects were related to the regulation of Th1/Th2 balance. At the same time the results indicate that it is reasonable for Yu-ping-feng used as powder in clinic.