目的:观察茵陈蒿汤细胞膜固相化色谱法特异性结合成分对肿瘤细胞的影响。方法:采用MTT法测定不同浓度的细胞膜固相色谱法得到的与肝细胞特异性结合的活性成分(样品A、B、C、D、E)对SMMC-7721人肝癌细胞及A549人肺癌细胞活力的影响,观察药物对癌细胞增殖的影响。结果:样品A、C、E能显著抑制SMMC-7721人肝癌细胞的增殖,样品A、B、C对人肺癌细胞A549具有显著抑制作用。结论:样品A、B、C、E可能是茵陈蒿汤抗肿瘤作用的活性成份。
Objective: To investigate the effects of the chemicals from Yinchenhao Decoction which specifically bind to hepatocytes on tumor cells.Methods: SMMC-7721 human hepatocellular carcinoma cells and A549 human lung carcinoma cells were used in this experiment.Cells were exposed to chemicals from Yinchenhao Decoction which specifically bind to hepatocytes(sample A,B,C,D,E).The proliferation of tumor cells were evaluated after 48h by MTT assays.Results: Sample A,C,E significantly inhibited SMMC-7721 cell proliferation.and sample A,B,C significantly inhibited A549 cell proliferation.Conclusion: Sample A,B,C,E maybe antitumor effective components of Yinchenhao Decoction.