中文摘要：

非核糖体肽具抗生素、抗病毒、抗癌及免疫抑制剂等重要的药用价值。以长松萝的地衣型真菌为研究材料，通过同源克隆和Gene walking的方法获取长松萝地衣型真菌全长非核糖体肽合成酶基因（UsNRPS），并对得到的UsNRPS进行生物信息学分析、分子系统进化分析及基因表达分析。结果表明：在地衣型真菌中，非核糖体肽在长松萝中为首次发现，BLAST比对表明其为NRPS基因的A结构域，生物信息学分析显示UsNRPS蛋白是一种非分泌蛋白，位于细胞质基质中；分子进化分析显示UsNRPS与埃默森蓝状菌（Rasamsonia emersonii）的NRPS基因的A结构域蛋白聚为一类；RT—PCR分析表明：2％肌醇、10％甘露醇、2％山梨醇、2％葡萄糖、10％葡萄糖、0．2％谷氨酰胺、1％甘氨酸、0．2％丙氨酸能够诱导NRPS基因的轻微表达，2％、10％蔗糖和果糖均能诱导NRPS基因的强烈表达；0．2％、1％的天冬氨酸均抑制NRPS基因的诱导表达。

英文摘要：

Taking Usnea longissima lichen were cloned by homology-based cloning and molecular phylogeny evolution and genetic forming fungi as the materials, gene walking approaches, and inducible expression were also the segment and full length of NRPS relevant analysis on its bioinformatics, conducted. For the lichen fungi, the non-ribosomal peptide was first reported inUsnea longissimi, and the BLAST comparison shows that it is at A （ adenylation） structure domain of the NRPS sgene. The bioinformatics analysis shows NRPS is a kind of secreted protein, which located in cytoplasmic matrix. The A domain proteins of NRPS gene of both UsNRPS and Rasamsonia emersonii were clustered. The RT-PCR analysis reveals that 2 % inositol, 10 % mannitol, 2 % sorbitol, 2 % glucose, 10 % glucose, 0. 2 % glutamine, 1% glacine, 0. 2 % alanine could induce the expression of NRPS slightly, 2 % , 10 % sucrose and fructose could lure the expression of NRPS heavily, while 0. 2 % , 1% asparagines could suppress the expression of NRPS.