中文摘要：

为研究不同碳氮源对牛樟芝菌丝体生长的影响,采用不同碳源（甘露醇、麦芽糖、山梨醇、蔗糖、乳糖、葡萄糖、肌醇、果糖、可溶性淀粉）、不同氮源（麦芽浸粉、酵母提取物、番茄浸粉、马铃薯浸粉、大豆蛋白胨、胰蛋白胨、牛肉浸粉、酪蛋白胨、麦芽浸粉肉汤）及不同浓度果糖（0．2%~3．2%）对牛樟芝菌丝体进行培养,观测其菌落大小、菌落长势、生长速率及菌丝生长指数。结果表明,以麦芽浸粉与酵母提取物为氮源,9种糖类中果糖作为碳源时,牛樟芝菌丝生长速度最快,培养30d时的菌丝生长速率为1．47mm/d,且菌丝呈红色,与野生牛樟芝子实体颜色接近。以麦芽糖和果糖为碳源,9种提取物中牛肉浸粉作为氮源时,牛樟芝生长最佳,培养30d时的菌丝生长速率为1．51mm/d,但其菌丝呈黄白色。果糖浓度在4%以内,生长的各个指标均随着果糖浓度的升高而升高。本研究为今后研究牛樟芝菌种扩繁、大规模发酵及椴木接种提供了实验依据。

英文摘要：

In order to studying the effect of different carbon sources and nitrogen sources on mycelia growth of Ant-rodia cinnamomea, 9 kinds of carbon sources and nitrogen sources were selected and control variable method were used to prepare the medium. The growth of mycelia on different carbon sources, different nitrogen sources and dif-ferent concentrations of fructose were observed. The result showed that using yeast extract as nitrogen source and u-sing maltose and fructose as carbon source can promote the growth of mycelium rapidly. The growth rate reached1. 47mm/d after 30 d’ s culturing, and this medium can make the color of mycelium change to be red. The different nitrogen sources experiments showed, using beef extract powder as a nitrogen source can significantly promote the growth of A. cinnamomea. The growth rate reached 1. 51mm/d after 30 d’s culturing, and the color of mycelium was white. When the concentrate of fructose were within 4 %, all relevant indicators increased with the increasing concentrate. This study provide a groundwork for expand breeding culture and large-scale fermentation of A. cinnamomea, and lay an important theoretical basis to inoculate on the basswood.