中文摘要：

目的：探讨敲除Attractin（Atrn）或Mahoganoid（Md）基因对雄性小鼠生殖内分泌激素的影响。方法：选取成年雄性Atrn基因敲除纯合子、Md基因敲除纯合子及正常小鼠（均为C3H/Hej种系）各12只设为Atrn基因敲除组、Md基因敲除组和对照组。内眦静脉取血清取外周血,化学发光法检测血清睾酮（T）、雌二醇（E2）浓度,用酶联免疫法（ELISA）检测血清卵泡刺激素（FSH）、黄体生成素（LH）和泌乳素（PRL）浓度,进行组间浓度对比。结果：Atrn基因敲除组和Md基因敲除组的外周血E2、LH、FSH、PRL浓度均低于对照组,差异有统计学意义（P〈0.05）;血清T浓度Atrn基因敲除组高于对照组,而Md基因敲除组低于对照组,差异无统计学意义（P〉0.05）。结论：Atrn或Md基因敲除后雄性小鼠的外周血E2、LH、FSH、PRL浓度均有下降,其具体机制及对外周血T浓度的影响有待进一步研究。

英文摘要：

Objective： To probe into the influence of the level of reproductive endocrine hormone of male mice after Attractin（Atrn） or Mahoganoid（Md） gene knockout. Methods： Male mature Atrn gene knockout homozygote mice, Md gene knockout homozygote mice and wild type mice（C3H/hej） were divided into 3 groups： Atrn knockout group, Md knockout group and control group. Each group had 12 mice. Blood samples were collected from angular vein of mice. The levels of serum T and E2 were determined with CLIA. The levels of serum FSH, LH and PRL were determined with ELISA. Results： The levels of serum E2, FSH, LH and PRL of Atrn knockout group and Md knockout group were lower than those in control group（P〈0.05）. The serum T level of Atrn knockout group was higher than that in control group（P〉0.05）. The serum T level of Md knockout group was lower than that in control group（P〉0.05）. Conclusion： The levels of serum E2, FSH, LH and PRL of male mice are down-regulated after Atrn or Md gene knockout. Its mechanism and whether the serum T level is affected need to be studied further.