中文摘要：

目的：探讨Attractin（Atrn）在培养大鼠睾丸leydig细胞的表达。方法：体外培养成年雄性sD大鼠睾丸leydig细胞，采用实时荧光定量RT-PCR检测Atrn mRNA表达，western blot检测Atrn蛋白的表达，同时应用化学发光法检测细胞睾酮分泌水平。结果：在体外培养大鼠睾丸leydig细胞过程中，Atrn mRNA、Atrn蛋白表达量和睾酮生成量逐渐减少。Atrn蛋白表达量与Atrn mRNA表达量（r=0．953，P〈0．05）和睾酮生成量（r=0．976，P〈0．01）成正相关。结论：大鼠睾丸Leydig细胞Atrn mRNA，Atrn蛋白表达量随培养天数的延长而减少，可能与睾酮的变化有关。

英文摘要：

Objective： To investigate the expression of Attractin （Atrn） in cultured adult rat Leydig cells. Methods： The gene and protein expression of Atrn in Leydig cells was quantitated by Real - time polymerase chain reaction （PCR） and western blot methods, respectively, while testosterone in the medium was detected by Chemiluminescence Immunoassay. Results： Expression levels of gene and protein of Atrn and the level of testosterone was decreased during culturing process. Multiple correlation analysis showed a positive correlation between the gene and protein expression levels of Atrn（r =0. 953, P 〈0.05）, and a positive correlation between the protein expression levels of Atrn and level of testosterone（r = 0. 976, P 〈 0.01 ）. Conclusion ： Expression level of Atrn is decreased in cultured Leydig cells, which may be related with testosterone production.