中文摘要：

目的探讨全血1干扰素诱导蛋白-10（IP-10）对活动性结核病的辅助诊断价值。方法检测66例活动性肺结核病患者、40例非结核呼吸疾病患者及40例健康对照者血浆中非特异性IP-10的水平；分别应用纯化的结核分枝杆菌特异性蛋白早期分泌性靶抗原-6（EAST-6）和培养液蛋白10（CFPl0）体外刺激患者全血，检测活动性结核病组、非结核呼吸疾病组和健康对照组人群全血中IP-10的释放水平；绘制受试者工作特征曲线，比较非特异性IP-10和特异性IP-10对活动性肺结核的诊断效能。结果活动性结核病组患者血浆中非特异性IP．10的水平为（139．6±124．2）pg／mL，明显高于健康对照组[（33．5±17．7）pg／mL，P〈0．05]；而与非结核呼吸疾病组[（88．1±73．3）pg／mL]无明显差别（P〉0．05）。经ESAT-6及CFPl0诱导后，活动性结核病组患者全血IP-10释放水平为（146．0±167．1）pg／mL，显著高于非结核呼吸疾病组[（26．6±9．7）pg／mL，P〈0．01]与健康对照组[（24．2±9．7）pg／mL，P〈0．01]。经过ROC分析，结核特异性的IP-10诊断结核病的临界值为41．2pg／mL，敏感度为68．2％，特异度为93．7％。特异性IP-10ROC曲线下面积为0．905，高于非特异性IP-10（0．747，P〈0．01）。结论IP-10可作为活动性结核病的辅助诊断方法。采用结核特异性IP-10较血浆非特异性IP-10能够提高活动性肺结核的诊断效能。

英文摘要：

Object ve To determine the clinical value of interferon-inducible protein 10 （IP-10） in auxiliary diagnosis of active tuberculosis. Methods The level of non-specific IP-10 was evaluated in the plasma of 66 active tuberculosis patients, 40 non-pulmonary tuberculous patients and 40 healthy controls, and the level of specific IP-10 was detected in the whole blood after stimulated by purified mycobacterium tuberculosis-specific antigen ESAT6 and culture filtrate protein 10 （CFP10）. Then the receiver operating characteristic （ROC） curve was drawn to determine the diagnostic value of non-specific IP-10 and specific IP-10 to detect active tuberculosis. Results The non-specific IP-IO level of the active tuberculosis group （139.6 ± 124.2 pg/mL） was obviously higher than that of the healthy control group （33.5 _± 17.7 pg/mL, P 〈 0.05 ）, but had no difference with that of the non-tuberculous pulmonary disease group （ 88.1 ± 73.3 pg/mL, P 〉0. 05 ）. After stimulated by the ESAT6 and CFP10, the specific IP-10 level of active tuberculosis group （ 146.0 ± 167.1 pg/mL） was signifieantly higher than that of non-tubereulous pulmonary disease group （26. 6 ± 9.7 pg/mL, P 〈0.01 ） and that of the healthy control group （24.2 ± 9.7 pg/mL, P 〈 0.01 ）. After ROC analysis, the threshold of the speeifie IP-10 for diagnosis of tuberculosis was 41.1 pg/mL and its sensitivity and specificity were 70.8% and 91.8% respectively. The area under curve （AUC） of the speeifie IP-10 was 0. 905, which was obviously higher than that of non-speeific IP-10 （0. 747, P 〈 0.01 ）. Conclusion The gamma interferon-indueible protein 10 release assay can be used as auxiliary diagnostic method for aetive tuber- culosis. The diagnostie value of the speeific IP-10 is better than that of non-specific IP-10.