中文摘要：

目的研究酸奶中乳酸菌对氨苄青霉素和四环素耐药性,对乳酸菌携带的抗性基因进行检测。方法从市售酸奶中分离并鉴定乳酸菌;采用纸片扩散法检测分离的菌株对四环素和氨苄青霉素的耐药性;设计引物,通过聚合酶链反应（PCR）扩增2种抗生素的耐药基因。结果从4种酸奶中分离鉴定出7株乳酸菌,均对氨苄青霉素或四环素耐药。PCR扩增得到5株菌携带对氨苄青霉素产生抗性的基因（Amp）为阳性,所有的菌株对四环素的耐药基因（Tet）均为阴性。结论部分对人体有一定生理功能的乳酸菌表现为耐药性,耐药性可以避免抗生素对菌体的杀灭,但是乳酸菌可能作为耐药性传播中间宿主应引起注意。

英文摘要：

Objective To investigate the ampicillin and tetracycline-resistant strains and resistant genes of lactic acid bacteria isolated from yogurt.Methods Lactic acid bacteria in yogurt were isolated and identified.Resistance to ampicillin or tetracycline of the strains were investigated with slip susceptibility test.Two pairs of primers were designed and ampicillin and tetracycline resistance gene were amplified.Results Seven lactic acid isolates were identified from 4 kinds of yogurt.A number of isolates were resistant to ampicillin or tetracycline.Five of the 7 lactic acid isolate contained ampicillin-resistant gene,and no isolate contained the tetracycline-resistant gene.Conclusion A number of lactic acid bacteria were resistant to antibiotics and could act as intermediate host of antibiotic resistant genes.