中文摘要：

目的明确铜绿假单胞菌噬菌体PaP3在感染宿主菌PA3后其全基因组的分时期表达模式。方法利用基因芯片检测噬菌体PaP3在感染宿主菌PA3后不同时间（5 min,10 min,20 min,30 min,80 min）其全基因组（含71个预测的ORF）在转录水平的表达谱变化,并进行非监督层次聚类（hierarchical clustering）分析。利用蛋白质合成抑制剂氯霉素（Cm）和DNA复制抑制剂磷酸乙酸（PAA）实验确定PaP3全基因组的分时期表达情况。结果根据时间表达模式的不同,PaP3全基因组可分为3大类：15个早期基因（ORF 71-57）、35个中期基因（ORF 56-22）及21个晚期基因（ORF 21-1）。在PaP3基因组结构中,这3类基因各自串联分布且可能受不同的操纵子调控。结论 PaP3感染过程中其早期、中期及晚期基因随时间有序表达,为深入了解噬菌体基因表达模式及生命复制周期奠定基础,并为了解噬菌体与宿主及机体免疫系统的的相互作用提供了基础。

英文摘要：

To investigate the transcription patterns of P.aeruginosa phage PaP3 in the host PA3 infection,we developed a viral DNA microarray containing 71 putative open reading frames of PaP3 to monitor the viral gene transcription program over the time course（5,10,20,30,80 min） of infection and to classify temporal kinetic expression classes of PaP3 transcripts by a de novo protein synthesis inhibitor（Cm） and a viral DNA replication inhibitor（PAA）.Result showed that the 71 PaP3 transcripts could be classified into three temporal kinetic classes： 15 early genes（ORF71-57）,35 intermediate genes（ORF56-22）,and 21 late genes（ORF21-1）.The three gene classes seemed to cluster in specific regions throughout the PaP3 genome.The early genes,intermediate genes and late genes of PaP3 were expressed in order of time.Our study illuminated the global transcription pattern of Pseudomonas aeruginosa phage PaP3 during infection,which will provide experimental foundation for better known of bacteriophage replicative cycle and the sophisticated interaction between bacteriophage and bacterium.