中文摘要：

目的 研究结核分枝杆菌感染对工型干扰素受体（IFNAR）基因表达的诱导作用及其在结核病患者外周血中表达的临床意义.方法 选取2013年1月至2013年5月在深圳市第三人民医院确诊的痰菌阳性的肺结核患者（TB组）15例;结核分枝杆菌潜伏感染（latent tuberculosis infection,LTBI）者（LTBI组）15例、健康对照（HC组）15例,均为同期医院体检人员.应用免疫磁珠从健康人外周血单个核细胞（PBMCs）中分选CD14＋单核细胞,在巨噬细胞集落刺激因子（M CSF）刺激下诱导分化为巨噬细胞,随后感染不同毒力结核分枝杆菌（H37Ra、H37Rv）,应用实时定量PCR检测巨噬细胞中IFNAR1和IFNAR2基因表达情况,以2-△△Ct值表示IFNAR1和IFNAR2基因拷贝数.观测HC组、LTBI组、TB组患者各15例,分析不同人群PBMCs中IFNAR基因表达的差异.采用GraphPad 4.0软件对数据进行统计处理.多组计量资料数据首先进行齐性检验以判断是否符合正态分布,若符合正态分布,则用-x±s表示,并通过方差分析进行两两比较,从而计算q值.计数资料进行“行×列”表x2检验,以P＜0.05为差异有统计学意义.结果 经H37Rv刺激后,IFNAR1基因表达水平（5.95±0.41）显著低于空白对照（7.53±0.41）,差异有统计学意义（q=4.15,P＜0.05）;而H37Ra刺激后IFNAR1基因表达水平为（6.54±0.33）,与空白对照（7.53±0.41）、H37Rv感染（5.95±0.41）相比差异均无统计学意义（q值分别为2.56、1.57,P值均＞0.05）;而IFNAR2基因在H37Ra和H37Rv刺激后表达水平分别为（8.81±0.85）、（8.84±0.80）,均显著低于空白对照组（12.67±1.15）,差异具有统计学意义（q值分别为4.10、4.13,P值均＜0.05）;而H37Ra和H37Rv刺激后IFNAR2的基因表达差异无统计学意义（q=0.02,P＞0.05）.IFNAR1基因在TB、LTBI、HC三组人群之间表达水平分别为（5.39±0.64）、（6.59±0.86）、（7.08±1.10）,两两比较差异均无统计学意义（TB vs HC

英文摘要：

Objective To explore whether Mycobacterium tuberculosis （Mtb） regulates the expression of IFNAR gene and its role in the patients with tuberculosis （TB）.Methods The cases were selected from Shenzhen Third People＇s Hospital during January to May 2013,involving sputum smear-positive TB patients（n=15）,the persons with latent tuberculosis infection （LTBI） （n =15） and healthy controls （HC） （n =15）.CD14＋ monocytes were isolated from peripheral blood mononuclear cells （PBMCs） and differentiated into human macrophages under macrophage colony stimulatory factor （M-CSF） stimulation,then expression of IFNAR1 and IFNAR2 genes was determined with qPCR assay after infection with Mtb H37Ra or H37Rv strains.All data was dealt with GraphPad 4.0 software.The homogeneity test of variances was used to judge whether multiple measurement data were in Gaussian distribution,if so,the data was shown as-x± s and analysis of variance（ANOVA）was used to compare multiple comparison to calculate q value.Enumeration data was compared with R× C Chi-square test.It was considered as significant difference with P＜0.05.Results IFNAR1 gene expression in the macrophages infected by Mtb H37Rv decreased significantly compared to the blank control （5.95±0.41 vs 7.53±0.41,q=4.15,P＜0.05）.IFNAR1 gene expression in the macrophages infected by Mtb H37Ra（6.54 ± 0.33） had not significant difference with the blank control （7.53±0.41）and H37Rv infection（5.95±0.41） （q=2.56 and 1.57,P＞0.05）.IFNAR2 gene expression had not significant difference in the macrophages infected by Mtb H37Rv （8.81 ± 0.85）or H37Ra （8.84 ± 0.80） （q 0.02,P＞0.05）,but they were significantly lower compared to the blank control（12.67± 1.15） （q =4.10 and 4.13,P＜0.05）.Furthermore,IFNAR1 gene expression had no significant difference among different groups （TB 5.39 ± 0.64;LTBI 6.59 ± 0.86;HC 7.08± 1.10,P＞0.05）,and IFNAR2 gene expression was lower in TB than that in HC（5.96 ± 0.58 vs 10