中文摘要：

为了揭示钾、硼对棉花叶片细胞膜及硼利用的影响，本试验以棉花作为研究材料，采用营养液培养方式，研究了不同钾、硼处理叶片逆境下细胞膜及与细胞壁结合的硼比例的变化。结果表明，施钾水平为20mg·L^-1时，缺硼（0mgB·L^-1）阻碍棉花地上部生长和干物质的积累，且与正常硼（0．2mgB·L^-1）相比，叶片逆境生理指标均显著升高，束缚态硼相对含量、R值（半束缚态硼／自由态硼）、细胞壁提取率和叶片总细胞壁硼占叶片硼的比例均有所升高；施钾水平为2mg·L^-1时，缺硼叶片逆境生理指标、自由态硼和半束缚态相对含量及R值较正常硼处理也均显著升高，但束缚态硼相对含量和叶片总细胞壁硼占叶片硼的比例降低。因此，适钾与低钾时，缺硼均会明显破坏细胞膜透性。适钾条件下，缺硼导致细胞壁增厚，在细胞壁与果胶多糖结合的硼的比例升高；而低钾时，缺硼对于叶片细胞壁提取率无显著影响，进入叶片细胞质的硼的比例升高，但与果胶多糖结合的硼的比例降低。

英文摘要：

To find out the effect of potassium（K） and boron （B） on cotton leaf cell membrane and B distribution and utilization, the membrane relative permeability, MDA, Pro, the content of free B, semi-bound B and bound B and the content of B in cell wall of cotton leaf were analyzed under different K levels with solution culture method in this study. The results showed that in normal K （20 mgK·L^-1）, B deficiency （0 mgB ·L^-1） hindered the normal growth and dry mass of shoots, in addition, the membrane relative permeability, the content of MDA and Pro significantly increased compared with the normal B（0.2 mgB·L^-1 ）, and the relative concentration of bound B, R value, cell wall material and the ratio of total cell wall B/leaf B increased by 10.32%, 21.28%; 31.35% and 31.35%, respectively. In contrast, under low K（2 mgK·L^-1） supply, B deficiency produced a very significant decrease in the relative concentration of bound B and the ratio of total cell wall B/leaf B. The above results showed that under either K-deficient or K-sufficient condition, B deficiency damaged the cotton leaf cell membrane and cell membrane permeability. In normal B-supplied plants, lacking of B induced more B into the cytoplasm, but increased the proportion of B that combined with the pectic polysaccharides in cell wall. However, under K-deficient treatment, the proportion of B cross-linked pectic polysaccharides in cell wall decreased.