中文摘要：

目的观察XAV939对卵巢癌细胞株OVCAR3增殖的作用。方法采用MTT法检测XAV939对卵巢癌细胞增殖的抑制作用,用流式细胞技术检测卵巢癌细胞株OVCAR3细胞周期和凋亡,Western blotting法检测Cyclin D1、Bcl-2的表达以及PARP剪切片段。结果 XAV939对卵巢癌细胞株增殖的抑制呈现浓度依赖性和时间依赖性,细胞周期检测表现为G1期阻滞,凋亡检测显示凋亡率增加。不同浓度的XAV939处理组的卵巢癌细胞Cyclin D1、Bcl-2蛋白表达水平下降,PARP剪切片段增多。结论 XAV939可以抑制卵巢癌细胞株OVCAR3的增殖。

英文摘要：

Objective To investigate the effects of XAV939 on the proliferation of ovarian cancer OVCAR3 cells in vitro. Methods The OVCAR3 ovarian cancer cells were cultured with different concentrations of XAV939. The cell proliferation was detected by MTT method, cell cycle and apoptosis were determined by Flow cytometry, the expression of Cyclin D1 and Bcl-2 proteins and the level of PARP cleavage segment were detected by Western blot. Results XAV939 inhibited the proliferation of OVCAR3 ovarian cancer cells with dose- and time-dependent manner, inhibited the cell cycle at G1 phase, and increased the apoptosis rate of the cells, and downregulated the expression levels of Cyclin D1 and Bcl-2 proteins, and upregulated the level of PARP cleavage segment in OVCAR3 ovarian cancer cells. Conclusion XAV939 can inhibit the proliferation of OVCAR3 ovarian cancer cells.