中文摘要：

目的观察含镁与无镁离子两种不同人工脑脊液对小鼠离体脑片谷氨酸损伤模型的影响。方法以培养的小鼠离体脑片为研究对象，分别用含镁离子和无镁离子人工脑脊液对脑片进行培养，不同浓度的谷氨酸（1、3mmol·L^-1）损伤30min，用TTC染色及酶标检测方法评价两种人工脑脊液对离体脑片谷氨酸损伤模型的影响。结果用含镁离子人工脑脊液孵育，空白对照组脑片TTC染色后抽提液490nm吸光度值为0．48±0．06，1、3mmol·L^-1谷氨酸损伤组吸光度值分别降到0．40±0．05（P〈0．05）、0．31±0．06（P〈0．01）。而在相同谷氨酸浓度水平，用无镁离子人工脑脊液孵育的脑片的活性均低于用含镁离子人工脑脊液孵育的脑片活性（P〈0．01）。结论 用两种人工脑脊液进行孵育，均可建立可靠的脑片谷氨酸损伤模型；但无镁离子人工脑脊液比含镁离子人工脑脊液对脑片损伤更为严重，人工脑脊液中的镁离子可能发挥了对脑片的保护作用。

英文摘要：

Aim To observe the effects of magnesiumcontained and magnesium-free ACSF on glutamate injury model in mice brain slices. Method Two kinds of ACSF, magnesium-contained and magnesium-free ACSF, were used to study glutamate injury in mice brain slices, and the viability of brain slices suffering from glutamate injury with two concentrations （1 mmol · L^-1 and 3 mmol· L^-1 ） for 30 min was evaluated by using TIC staining method. Result When brain slices were incubated with magnesium-contained ACSF, the 490 nm absorbance of the extracted liquid of TIC staining for brain slices of the control group was 0. 48 ± 0. 06, and that of the 1 mmol· L^-1 and 3 mmol· L^-1 glutamate injury group dropped to 0. 40 ＋ 0. 05 （P 〈 0. 05 ） and 0. 31 ±0. 06 （ P 〈 0. 01 ） respectively. And the viability of brain slices incubated with magnesium-contained ACSF was constantly worse than that incubated with magnesium-contrained ACSF when glutamate of the same concentration added. Conclusion Credible glutamate injury model can be established by incubating brain slices with both the two ACSF, and magnesium-free ACSF produces a severer injury on brain slices compared with magnesium-contained ACSF, in which magnesium probably exerts protective effect on brain slices.