中文摘要：

目的探讨氯化铯对大鼠学习记忆功能及突触可塑性的影响。方法大鼠腹腔注射氯化铯30d（120mg·kg^-1，每天一次）建立一种动物模型，然后运用在体电生理技术记录该模型大鼠海马CA3区群峰电位，给予高频刺激后观察诱发长时程增强（LTP）的情况，同时观察对照组大鼠（腹腔注射生理盐水）的LTP诱导情况；运用Mor6s水迷宫检测不同组大鼠空间学习记忆能力；在电镜下观察各组动物海马CA3区超微结构的改变；运用高效液相色谱法测定不同组大鼠海马组织内兴奋性氨基酸和抑制性氨基酸的含量。结果①氯化铯组的LTP诱导率为33、33％，对照生理盐水组为100％；②Morris水迷宫检测发现氯化铯组学习记忆能力受到一定程度的损害。学习训练结束时，氯化铯造模组较对照组逃避潜伏期延长；空间探索实验发现，氯化铯造模组、生理盐水组在原平台所在象限穿梭时间占探索总时间百分比分别为（14．76±2．87）％、（38，58±6．99）％；③电镜下观察到经高频刺激后生理盐水组CA3区突触结构中突触前膜的突触小泡明显比正常大鼠未经过高频刺激组增多，而氯化铯组经过高频刺激后CA3区突触结构中突触前膜的突触小泡较生理盐水组经过高频刺激后突触小泡的数量显著减少；④海马组织氨基酸含量的改变：NS组大鼠经高频刺激后，海马组织谷氨酸和γ-氨基丁酸、天冬氨酸、甘氨酸以及牛磺酸的含量均明显高于未经过高频刺激的正常对照组（Normal组）（P值均小于0．05），而CsCl组大鼠经过高频刺激后上述5种氨基酸含量均少于经过高频刺激后的NS组，但有谷氨酸、γ-氨基丁酸和甘氨酸有显著性差异（P〈0．05），而天冬氨酸和牛磺酸含量则没有显著性差异。结论氯化铯可使大鼠的空间学习记忆能力受到明显损害，而且阻碍LTP现象的诱导，其作用机制可能为通

英文摘要：

OBJECTIVE To study the effects of CsC1 on synaptic plasticity , spatial learning and memory. METHODS Rats were injected intraperitoneally with CsC1 （120 mg · kg^-1 ,once per day for 30 d） ,then the evoked population spike（PS） was recorded in hippocampus CA3 region in vivo, After the induction of PS, long-term potentiation （LTP） was induced with high frequency stimulation（HFS）. Spatial learning and memory performance in different groups rats were measured by Morris water maze. Ultrastructural pathologic features of the CA3 region of hippocampus were invesgigated by electron microscopy. The contents of glutamate（ GLU）, aspartate （ASP）, glycine（GLY） , gamma-aminobutyric acid （GABA） and taurine （TAU） in hippocampus were measured by HPLC. RESULTS ①The induction rate of long-term potentiation （LTP） in the CsCI group was lower than those in NS group, which was 33.33% in CsCI group,and was 100% in NS group. ②CsC1 resulted in spatial learning and memory impairments shown by longer escape latency and shorter time spent in the target quadrant. ③Under the electron microscopy, the synaptic vesicles in the CsCI were obviously decreased （in control group） compared with those in NS group. ④The amounts of glutamate（ GLU）, aspartate（ ASP）, glycine （GLY） , gamma-aminobutyric acid （GABA） and taurine（TAU） in hippocampus in NS group showed significant difference compared with the normal rats without high frequency stimulation, which were significantly increased compared with the normal group （P 〈 0. 05 for all）, but the amounts of the five amino acids in CsCI group were all reduced compared with NS group. In CsC1 group,the amounts of GLU, GLY, GABA showed significant difference （P 〈 0.05 for all）, but the amounts of ASP and TAU showed no significant difference. CONCLUSION CsCI impaired the induction of LTP, the spatial learning and memory. The mechanism was suspected to block the hyperpolarization -activated cyclic nucleotide-gated channel（HCN）.