中文摘要：

建立实时荧光的方法的目的为在鼻咽的癌(NPC ) 检测 Survivin mRNA 的表示的量的 RT-PCR 纸巾。全部的 RNA 从 NPC 房间被提取的方法与 Trizol 衬里 CNE-2 和纸巾然后相对地抄录了到 cDNA ，为在 NPC 纸巾检测 Survivin mRNA 的表示的量的 RT-PCR 被建立了的实时荧光的一个方法，在哪个长期的鼻咽炎病人当作控制的鼻咽纸巾组织。结果 Survivin mRNA 的表示也都能在 CNE-2 房间被检测， NPC 纸巾或在长期的鼻咽炎，病人是鼻咽纸巾，并且有更高，表示比在长期的鼻咽炎，病人比哪个是鼻咽纸巾在 NPC 纸巾 Survivin 铺平 mRNA ，差别是重要的( P 【 0.01 )。Survivin mRNA 的表示能在舞台两个都被检测我 + II 和阶段 III + IV NPC，并且在在这二个组之间的基因表示的相对数量化离子没有有效差量(P 】 0.05 ) 。在 NPC 病人的 Survivin mRNA 表示和年龄和性别之间没有关系(P 】 0.05 ) 。结论实时荧光量的 RT-PCR 是为在 NPC 纸巾检测 Survivin mRNA 的表示的一个快速、有效、高的敏感方法。过去 Survivin mRNA 的表示可以在 NPC 的致病起一些作用。

英文摘要：

Objective： To establish the method of real time fluorescence quantitative RT-PCR for detecting the expression of Survivin mRNA in nasopharyngeat carcinoma （NPC） tissues. Methods： The total RNA was extracted from NPC cell line CNE-2 and tissues with Trizol and then been transcribed reversely to cDNA, a method of real time fluorescence quantitative RT-PCR for detecting the expression of Survivin mRNA in NPC tissues had been established, in which chronic nasopharyn-gitis patients＇ nasopharynx tissues treated as control group. Results： The expression of Survivin mRNA all could be detected either in CNE-2 cells, NPC tissues or in chronic nasopharyngitis patients＇ nasopharynx tissues, and there was higher the expression level of Survivin mRNA in NPC tissues than which in chronic nasopharyngitis patients＇ nasopharynx tissues, the difference was significant （P 〈 0.01）. The expression of Survivin mRNA could be detected both in stage Ⅰ ＋ Ⅱ and stage Ⅲ ＋ Ⅳ NPC, and there was no significant difference in relative quantifications of gene expression between these two groups （P 〉 0.05）. There was no relationship between Survivin mRNA expression and age and sex of NPC patients （P 〉 0.05）. Conclusion： Real time fluorescence quantitative RT-PCR is a rapid, effective and high sensitive method for detecting the expression of Survivin mRNA in NPC tissues. The overexpression of Survivin mRNA may play some roles in pathogenesis of NPC.