中文摘要：

目的研究灯盏花素经Caco-2细胞模型转运的特性。方法用培养于Transwell上的Caco-2单层细胞模型研究灯盏花素双向转运；采用生物活性测定及HPLC的方法测定介质中灯盏花素或灯盏乙素的转运量。结果两种方法测定结果显示灯盏花素与灯盏乙素的双向转运Papp具有高度一致性，两者Papp（A—B）均小于1×10^-6cm·s^-1，流出率ER均大于2。结论采用生物活性法测定灯盏花素Papp具可行性。灯盏花素在Caco-2细胞单层吸收上存在明显外排，这可能是其生物利用度极低的重要原因。

英文摘要：

Aim To evaluate transport property of breviscapine across Caco-2 monolayer model by different assay methods. Methods The transwell filter inserts on culturing Caco-2 cell monolayers was used to observe breviscapine bidireetion transportation; bioactivity and HPLC approach were used to determine breviscapine and scutellarin in the receiver compartment. Results There was a good degree of concordance between breviscapine and seutellarin Papp values for apical（AP） to basolateral （BL） or BL to AP, both Papp values for AP-BL were less than 1×10^-6 cm·s^-1. The efflux rates of breviscapine and scutellarin were larger than 2. Conclusions It is feasible to determine Papp, of breviscapine by bioactivity assay method. Breviscapine is obviously secreted by Caco-2 cell monolayer, which is a possible important reason for low oral bioavailability of breviscapine.