中文摘要：

目的观察肉苁蓉多糖（CDPS）对巨噬细胞的活化作用。方法采用中性红法测定吞噬活性；Griess试剂法测定NO释放量；L929细胞法及小鼠胸腺细胞法测定TNF—α及IL-1释放。结果CDPS在6．25～50mg·L^-1剂量范围内可剂量依赖性地增强BALB／c小鼠腹腔巨噬细胞吞噬中性红能力和促进NO释放；在2．8～100mg·L^-1剂量范围内可使正常和免疫抑制的RAW264．7细胞NO释放明显增加；在0．56～7．2mg·L^-1或3．6～10mg·L^-1范围促进RAW264．7细胞TNF—α或IL-1产生，均具有良好的量效关系。结论CDPS可明显提高巨噬细胞吞噬及分泌功能，活化巨噬细胞。肉苁蓉免疫增强作用可能与此有关。

英文摘要：

Aim To investigate the activation effect of Cistanche deserticola polysaccharides （ CDPS ） on marcrophages. Methods The phagocytic ability of peritoneal macrophage （MФ） was evaluated with neutral red dye phagocytosis assay;nitric oxide （NO） production was examined by Griess reaction;the activity of tumor necrosis factor-α （TNF-α） and interleukin-1 （ IL- 1 ） secreted were measured by L929 cells bioassay and proliferation of mouse thymocyte. Results CDPS at the concentration range of 6. 25 -50 mg.L^-1 enhanced the phagocytic ability and NO release of peritoneal macrophage in a dose-dependent manner. CDPS at the dose of 2.8 -100 mg . L^-1 significantly increased NO secretion of normal or immunesuppressed RAW264.7 cells. TNF-α production by RAW264. 7 cells was promoted after incubation of RAW264.7 cells with CDPS at the concentration of 0. 56 - 7.2 mg.L^-1. IL-1 release of RAW264. 7 eells was obviously elevated by CDPS at the dose of 3.6 - 10 mg.L^-1 , and they both showed a good dose-effeet relationship. Conclusions CDPS can markedly promote the phagocytic and secretory functions of MФ, and activate MФ, which suggests that the immunomodulatory effect of CDPS may be related to it.