分别配置盐度为13、18、23、28、33、38和43的海水, (23±0.5)℃温度条件下孵化拟目乌贼(Sepia lycidas)卵, 并对拟目乌贼胚胎发育情况进行观察与分析。结果表明, 拟目乌贼胚胎在盐度为28时发育最佳, 孵化历期为28-30天, 胚胎发育经过受精卵、卵裂期、囊胚期、原肠胚期、胚芽原基形成期、胚体形成期、漏斗形成期、眼点形成期、红眼期、心跳出现期、内壳发生期、色素形成期、墨囊形成期、黑眼期、内壳形成期和幼体出膜期等16个典型时期。盐度为13、18、38和43时, 发育停滞, 说明海水盐度是拟目乌贼胚胎发育过程中一个很重要的影响因素。
To understand the salinity impact on embryonic development of cuttlefish Sepia lycidas, we carried out an experiment at water temperature (23±0.5)℃ in pH 7.41 in salinities of 13, 18, 23, 28, 33, 38 and 43. The results show that the most suitable salinity was 28, and the peak time of hatching was 28-30d. The embryonic development was divided into 16 stages: cleavage stage, blastula stage, gastrulae stage, organ development stage, embryo growth stage, and so on. The embryonic development failed at salinity 13, 18, 38 and 43. Therefore, the salinity is an important environmental factor for S. lycidas early development.