中文摘要：

我们开发了一个种类特定的 PCR 方法在 10 海黄瓜种类的使脱水的产品之中识别种类。在有一份向前通用的教材的联合，从 16S rRNA 基因设计的十份反向的种类特定的教材产生了 ca 的 PCR 碎片。为每种的 270 bp 长度。PCR 试金的特性与 21 海黄瓜种类的样品的 DNA 被测试。扩大仅仅在特定的种类被观察。我们开发了的种类特定的 PCR 方法成功地被使用证实使脱水的海黄瓜的商业产品的种类，并且被证明是一种有用、快速、便宜的技术识别海黄瓜产品的起源。

英文摘要：

We developed a species-specific PCR method to identify species among dehydrated products of 10 sea cucumber species. Ten reverse species-specific primers designed from the 16S rRNA gene, in combination with one forward universal primer, generated PCR fragments of ca. 270 bp length for each species. The specificity of the PCR assay was tested with DNA of samples of 21 sea cucumber species. Amplification was observed in specific species only. The species-specific PCR method we developed was successfully applied to authenticate species of commercial products of dehydrated sea cucumber, and was proven to be a useful, rapid, and low-cost technique to identify the origin of the sea cucumber product.