中文摘要：

目的：观察反油酸对人脐静脉内皮细胞（HUVEC）增殖活力的影响。方法：采用50、100、200、400、600μmol/L等5个浓度的反油酸作用于人脐静脉内皮细胞,以正常组为阴性对照组,NaOH组（200μmol/L）为阳性对照组,MTT法和台盼蓝排斥实验检测其对内皮细胞的毒性。结果显示：反油酸组内皮细胞增殖活力明显低于正常组,且高浓度组（400、600μmol/L）对细胞增殖抑制率明显高于低浓度组（50μmol/L）;而NaOH组与正常组比较却无明显差异。此外,反油酸组活细胞率降低。结论：反油酸对内皮细胞有明显毒副作用,其生长抑制率与反油酸浓度和作用时间呈正相关。

英文摘要：

Objective：To observe the effect of oleic acid on the proliferation of human umbilical vein endothelial cells （HUVECs）. Methods：HUVECs were treated with oleic acid at concentrations of 50,100,200,400μmol/L and 600 μmol/L （in combination with NaOH at a final concentration of 200 μmol/L）,respectively. The normal group receiving no treatment was served as the negative control,and the groups treated with NaOH at 200μmol/L alone were used as the positive control. MTT assay and trypan blue exclusion tests were used to evaluate the toxicity of oleic acid on cells. Results：Compared with the normal group,oleic acid significantly inhibited the proliferation of HUVECs. In addition,compared with the group treated with oleic acid at a low concentration （50μmol/L）,an obvious strong inhibition effect on cell proliferation was observed in groups treated with oleic acid at high concentrations （400 μmol/L and 600 μmol/L）. However,no significant difference was observed between the positive control and the normal group. Moreover,the amount of viable cells in oleic acid-treated groups was obviously reduced. Conclusion：Oleic acid has obvious side effect on HUVECs in a concentration-and time-dependent manner.