中文摘要：

目的研究铁缺乏对大鼠甲状腺细胞系（fisher rat thyroid cell line,FRTL）甲状腺过氧化物酶表达的影响,并探讨其影响机制。方法体外培养FRTL细胞,用原子吸收火焰法测定F-12完全培养基中的铁含量,根据其完全培养基的铁含量分为4组：正常对照组（0.20μg/ml）,轻度铁缺乏组（0.10μg/ml）,中度铁缺乏组（0.05μg/ml）,重度铁缺乏组（0.00μg/ml）,培养48 h后,用实时定量聚合酶链反应和细胞免疫荧光技术对不同铁干预条件下甲状腺过氧化物酶（thyroid peroxidase,TPO）mRNA及蛋白表达进行检测。结果随着培养液中铁含量的降低,FRTL细胞TPO mRNA表达水平逐渐减少,且中、重度铁缺乏组与对照组比较,差异均有统计学意义（均有P〈0.05）。铁缺乏组TPO蛋白较对照组表达减少且荧光强度较弱。结论铁缺乏降低甲状腺过氧化物酶的表达可能是导致甲状腺功能低下的一个重要机制。

英文摘要：

Objective To study the impact of iron deficiency on the expression of thyroid peroxidase （TPO） by fisher rat thyroid cell line （FRTL） ceils and to explore the interaction mechanism. Methods FRTL cells were cultured in vitro. The concentrations of iron of F-12 completely culture medium were determine by flame atomic absorption. The experiment results were divided into four groups according to the concentrations of iron of completely culture medium, which were control group （0. 20 μg/ml）, mild iron deficiency group （0. 10 μg/ml）, moderate iron deficiency group （0. 05 μg/ml） and severe iron deficiency group （0. 00 μg/ml） respectively. The FRTL cells were treated with 0. 20 μg/ml, 0. 10 μg/ml, 0. 05 μg/ml, 0. 00 μg/ml iron for 48 hours, respectively. The expressions of mRNA and protein of TPO in the FRTL were assessed by real-time polymerase chain reaction（PCR） and immunofluorescence techniques. Results The mRNA expression of TPO in the FRTL gradually decreased with the decreasing concentrations of iron in medium. At the same time, compared with control group, the mRNA expression of TPO was different in moderate and severe iron deficiency groups, and the difference was statistically significant （ both P 〈 0. 05）. The expression of TPO protein were lower in iron deficiency group than in control group. Conclusions Iron deficiency reduces thyroid function , which perhaps is due to the reduction of expression of TPO.