中文摘要：

目的观察碘铁联合缺乏对大鼠甲状腺功能的影响，为缺铁地区的碘缺乏病防治工作提供新的线索和思路。方法SPF／VAF级初断乳SD雄性大鼠60只．按体质量采用2×2析因分析方法将大鼠随机分为对照（N）组、碘缺乏（ID）组、铁缺乏（FD）组、碘铁联合缺乏（IFD）组，每组15只。通过饮食控制动物碘、铁摄入量，各组均自由饮用去离子水。喂养6周后，称取大鼠体质量和甲状腺质量；采集大鼠尿样，过硫酸铵消化-砷铈催化分光光度法检测大鼠尿碘；股动脉取血，生化法检测全血血红蛋白、血清铁水平和总铁结合力；化学发光免疫分析法检测血清总三碘甲腺原氨酸（TT3）、游离三碘甲腺原氨酸（FT3）、总甲状腺素（TT4）、游离甲状腺素（FT4）和促甲状腺激素（TSH）。结果与N组[（341．0±24．2）g]比较，FD组[（307．0±17．6）g]和IFD组[（294．5±29．5）g]大鼠体质量减轻（P均〈0．01）；ID组和IFD组大鼠甲状腺绝对质量[（31．8±3．8）、（22．9±3．4）mg]和相对质量[（9．4±1．6）、（7．8±1．1）mS／100g体质量]均高于N组[（17．6±3．7）mg，（5．2±1．I）ms／100g体质量，P均〈0．01]。大鼠尿碘中位数IFD组和ID组（22．5、31．5t．Lg／L）明显低于FD组和N组（167．6、163．8μg／L，P均〈0．01）。IFD组和FD组血红蛋白[（69．2±7．4）、（62．2±8．1）g／L]和血清铁[（4．9±1．4）、（5．2±0．8）la,mol／L]低于N组[（161．1±9．4）g／L，（27．3±6．5）μmol／L，P均〈0．01]，总铁结合力[（176．3±4．4）、（186．4±13．7）I,Lmol／L]高于N组[（99．1±9．0）μmol／L，P均〈0．01]。FD、ID、IFD组的皿[（47．42±6．58）、（35．27±6．97）、（30．07±4．75）nmol／L]、FT4[（27．60±2．32）、（21．67±2．79）、（19．45±1．97）pmol／L]低于N组[（68．97±9．95）nmol/L，（34．01±3．28）pmol／L，P均〈0．01

英文摘要：

Objective To explore the effect of iodirle and iron comitant deficiency on thyroid function of rat, and to provide new clues and ideas for prevention and control of iodine deficiency disorders in iron deficiency areas. Methods Using 2× 2 factorial analysis method, sixty male healthy SPF/VAF level weaning SD rats were randomly divided into normal group （N）, iodine deficiency group （ID）, iron deficiency group （FD） and iodine and iron comitant deficiency group（IFD） by body mass, and 15 rats in each group. Diet was controlled to meet the iodine and iron intake requirement in each group. Rats of all groups drank deionized water. After 6 weeks feeding, body mass and thyroid gland mass were measured, and the relative mass of thyroid gland was calculated. Rat urine samples were collected, （NI-I4）2S208 digesting, As3±-Ce4± catalyzing spectrophotometry was used to verify urine iodine. Blood was taken from femoral artery, and serum was obtained by centrifugation. The following tests were carried out： biochemical assay of hemoglobin, serum iron, and total iron binding capacity ; chemiluminescence detection oftotal triiodothyronine （TY3）, total thyroxine （TT4）, free triiodothyronine （FT3）, free thyroxine （FT4） and thyroid stimulating hormone （TSH）. Results Compared with N group [ （341.0 ± 24.2）g], rats in FD and IFD groups [ （307.0 ± 17.6）, （294.5 ± 29.5）g ] had less body mass （all P 〈 0.01 ）; and compared with N group[（ 17.6 ± 3.7）mg, （5.2 ± 1.1）mg/100 g body mass] those in ID and IFD groups had .larger absolute[ （31.8 ±3.8）, （22.9 ± 3.4）mg] and relative thyroid mass[ （9.4 ± 1.6）, （7.8 ±1.1 ）mg/100 g body mass, all P 〈 0.01 ]. Medians of urinary iodine in ID and IFD groups（31.5, 22.5 μg/L） were lower than that in FD and N groups（167.6, 163.8 μg/L, all P 〈 0.01）. Rats in IFD and FD groups had lower hemoglobin[ （69.2 ± 7.4）, （62.2 ± 8.1）g/L] and serum iron[ （4.9 ± 1.4）, （5.2 ± 0.8） ~mol/L ] and high