中文摘要：

目的观察在双酚A（BPA）的作用下,CD1小鼠肝细胞凋亡是否受到影响。方法 48只4周龄雄性CD1小鼠随机分成4组（12只/组）,分别予以含不同浓度BPA[0、5、50和500μg/（kg·d）]的标准饲料喂养8周后,利用HE染色、Hoechst 33258染色观察肝脏组织形态学及细胞凋亡,Western blot法检测肝脏组织中cleaved caspase-3、Bax和Bcl-2蛋白表达,用分光光度法测定caspase-3酶活性。结果与对照组相比,BPA各处理组肝脏组织中凋亡细胞明显增多（P〈0.01）,肝脏组织促凋亡基因cleaved caspase-3与Bax蛋白表达增加（P〈0.05）;caspase-3酶活性显著增强（P〈0.01）。结论在环境内分泌干扰物BPA的作用下,CD1小鼠肝细胞凋亡增加。

英文摘要：

Objective To investigate the effect of BPA on hepatocyte apoptosis in CD1 mice. Methods Forty-eight4-week old male CD1 mice were randomly divided into 4 groups. Mice from different groups were exposed to different doses of BPA[0,5,50,500 μg /（ kg·d） ]through food contamination for 8 weeks. After the decapitation of mice and the isolation of liver,HE staining and Hoechst 33258 staining were used to observe the hepatocyte apoptosis; Western blot was performed to determine the protein expression of cleaved caspase-3,Bax and Bcl-2 in liver;caspase-3 activity was detected with a caspase-Glo3 /7 assay kit. Results Compared with the control group,mice treated with different concentrations of BPA showed an enhanced cell apoptosis and increased caspase-3 activity in liver（ P 〈0. 01）,with significantly increased protein expression of cleaved caspase 3 and Bax（ P〈0. 05）.Conclusions BPA induces hepatocyte apoptosis of CD1 mice.