中文摘要：

基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱技术能高效解吸三七提取液（Panaxnotoginseng Extraction，PNE）中的混合皂甙分子为皂甙离子，并供质量分析器检测与分析。选用MALDI-TOF质谱技术直接分析色谱纯皂甙样品的纯度，其检测灵敏度优于反相高效液相色谱法（RP-HPLC）。优化提取中药三七（Panax notoginseng，PN）的混合皂甙，选用MALDI-TOF质谱技术直接分析PNE中的皂甙种类和相对含量，发现PNE至少含有20种不同分子结构的皂甙组分，其中人参皂甙（Ginsenoside）Rg1和三七皂甙（Notoginsen-oside）R1含量相对较高。选用薄板层析法（TLC）制备PNE中的R1皂甙。MALDI-TOF质谱技术研究蓝斑背肛海兔（Notarcus leachiicirrosus Stimpson，NLCS）神经连索内的超微量R1的组成与分布。建立PNE皂甙的指纹图谱，并适合于评价中药三七的品质和分析体内超微量皂甙的代谢过程与机理。

英文摘要：

Mixed saponin molecules in the extraction of Panax notoginseng（PNE） can be effectively desorpted into the molecular ionization for measurement and analysis by mass analyzer from matrix assisted laser desorption ionization-time of flight-mass spectrometry（MALDI-TOF MS）. The saponin samples with chromatography purity were directly analyzed by MALDI-TOF MS, which indicated that the sensitivity of the method was higher than that of RP-HPLC. A technology of MALDI-TOF MS was directly employed to analyze the saponin kinds and their relative contents in Panax notoginseng（PN） while the saponins were perfectly extracted from the Chinese traditional medicine of PN. It was indicated that there were at least 20 saponins consisting of different molecular structure and that the content of both ginsenoside Rgl and notoginsenoside R1 in PNE was relative high. R1 saponin was extracted and identified to follow its metabolism pathway by both thin layer chromatography and MALDI-TOF MS, respectively. The saponin fingerprinting maps in PNE can be established to evaluate the quality of PE and to study both metabolism pathway and mechanism of extra minim saponins in vivo.