中文摘要：

为了得到具有核酸切割功能的人工核酸酶,设计合成了二乙烯三胺单齿、双齿、四齿配体,目标化合物的结构经红外、核磁氢谱、碳谱和电喷雾质谱确认。在磷酸氢钠-磷酸二氢钠缓冲溶液中对质粒DNA切割活性的初步实验结果表明：在多齿配体的锌（Ⅱ）配合物存在下,37℃保温48 h后,质粒DNA由Form Ⅰ断裂为Form Ⅱ,即合成的目标化合物有明显的切割活性。通过T4连接酶实验证明了目标化合物对DNA的切割为水解切割机制。

英文摘要：

A series of multinuclear diethylenetriamine ligands were synthesized and used as artificial nuclease enzyme model.Target compounds were characterized by 1H NMR,13C NMR,IR and ESI-MS.Preliminary studies on the cleavage of pUC19 DNA in the presence of metal complexes have also been performed and the results revealed that these complexes could act as powerful catalysts for the cleavage of pUC19 DNA after 48 h under physiological conditions.The hydrolytic cleavage mechanism of DNA plasmid by title compound was confirmed by T4 DNA ligase experiment.