中文摘要：

目的：提取斜带髭鲷高质量基因组DNA并建立适用于AFLP分析的体系。方法：以斜带髭鲷（Hapalogenys nitens）为材料提取高质量基因组DNA,并进行AFLP扩增。对AFLP分析体系中几个关键环节（基因组双酶切、连接、预扩增及选择性扩增、变性聚丙烯酰胺凝胶电泳和银染）做了优化。结果：提取的斜带髭鲷全基因组DNA纯度高,无拖尾。采用9组选择性引物共检测出350个不同的扩增位点,其中多态位点为175个,多态性比例为49.58%。聚丙烯酰胺凝胶电泳图像染色均匀,条带清晰且无背景干扰。结论：该分析体系的建立为斜带髭鲷的群体遗传多态性、种质资源、遗传图谱、遗传育种等研究奠定基础。

英文摘要：

Objective：To extract high-quality genomic DNA and establish a suitable for amplified fragment length polymorphim（AFLP） analysis system of Hapalogenys nitens.Method：We used AFLP technique to carry out the high-quality DNA which had extracted,and optimized several key procedures of AFLP including enzyme-digested reaction,ligation,pre-amplification selective amplification,PAGE and silver-stainin.Result：The genome DNA extracted from Hapalogenys nitens was high purity and had little trailing.9 selective primers amplified a total of 350 sites,of which polymorphic loci was 175,proportion of polymorphic loci is 49.58%,polyacrylamide gels is clear and dyeing uniformity without background interference.Conclusion：The establishment and optimize of AFLP analysis system in this paper would lay a groundwork for the research of Hapalogenys nitens genetic diversity,germplasm resource,construction of genetic linkage map,genetic breedin and so on.