中文摘要：

目的建立刺梨的高效液相色谱（HPLC）特征图谱测定方法。方法采用反相高效液相色谱法，HanbonPhecdaC18柱（250mm×4．6mm，5μm），乙腈（A）-0．05％磷酸溶液（B）为流动相，梯度洗脱，流速1mL·min-1，检测波长255nm，柱温：25℃；测定了14批刺梨药材的指纹图谱，应用相似度分析和聚类分析方法对药材进行分类，建立指纹图谱共有模式。结果建立了刺梨药材的指纹图谱测定方法及其特征图谱，不同产地药材图谱具有一定差异性。结论刺梨HPLC特征图谱的建立为药材质量控制提供了科学依据。

英文摘要：

Objective To establish an HPLC method for determining fingerprint of Rosa roxbughii. Methods The HPLC method was used with Hanbon Phecda C18 column（250 mm × 4.6 mm,5 μm）. The mobile phase consists of acetonitrile （A） and 0.05% phosphoric acid （B） with a gradient elution. The column temperature was set at 25℃, and the detective wavelength was 255 rim. The HPLC fingerprint for 14 batches of Rosa roxbughii was studied. The similarity analysis and cluster analysis were taken to classify the herbs and establish a common pattern of fingerprints. Results A HPLC method for determination of ingredients in Rosa roxburghii tratt and the fingerprint were established. A unique fingerprint was presented for herbs from different areas. Conclusion The establishment of HPLC feature fingerprint of Rosa roxburghii Tratt provides scientific basis for herbs quality control.