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中文摘要：
微生物膨胀素在生物炼制行业具有重大潜在应用前途。开展了从重组里氏木霉膨胀素生物学特性测定的工作。研究了发酵液中膨胀素的生物活性，初步的生物活性测定显示，该蛋白单独使用不具水解纤维素活性．但在纤维素酶水解纤维素时表现出明显的协同活性。结果表明，温度和pH等因素对分离得到的swollenin蛋白活性影响较大，并确定了最适的pH和温度范围。
英文摘要：
Swollenin is a kind of very important protein for biomass utilization in the biorefinery engineering. The recombinant Aspergillus oryzae was constructed to produce Trichoderma reesei swollenin. The preliminary bioactivity assay showed that the swollenin could not hydrolyzing cellulose mocrofibrils but could exhibit a significant synergistic effect on cellulose hydrolysis with cellulose. Further studies showed that the bioactivity of swollenin was affected by different temperature and pH, and the optimal ranges ofpH and temperature were determined for its full bioactivity.
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