中文摘要：

研究原始管道系统（PVs）与经络之间的关系。采用台盼蓝标记PVs,观察到不同的性别、年龄以及麻醉药的注射方式对大鼠腹腔表面PVs出现的影响。在大肠杆菌造成腹腔炎症后,PVs的出现率达到100%（n=20）,而对照组仅为10%（n=20）。采用DAPI、Phalloidin荧光染色结合CD11b和ICAM-1免疫荧光标记对PVs的结构进行了研究,观察到在正常大鼠和腹膜炎大鼠的PVs中均为CD11b和ICAM-1荧光免疫阳性标记,主要为成纤维细胞和白细胞。腹腔炎症大鼠PVs中DAPI标记的多形核和细胞膜上CD11b、ICAM-1阳性标记的细胞膜和胞浆较正常大鼠的PVs明显增多。表明PVs的出现与炎症密切相关。在功能方面,不同强度电刺激腹腔脏器与腹壁相连的PVs,对胃运动没有影响。针刺中脘穴,在PVs保留完整和剪除后均可引起胃运动的抑制;同样针刺足三里穴,在PVs保留完整和剪除后均可引起胃运动的增强。结果表明PVs并不参与针刺对胃肠运动的调节效应。因此PVs明显与指导针灸临床的经脉-脏腑相关理论不符。

英文摘要：

The relationship between the primary vascular system （PVs） and acupuncture meridians was investigated. The effects of different sexes, ages and anesthetic injection modes on the occurrence of rat peritoneal surface PVs were observed by trypan blue staining of PVs. The occurrence of PVs reached 100% after colon bacillus-induced peritonitis （n=20） and only 10% in the control group （n=20）. DAPI fluorescence staining and fluorescent phalloidin staining were combined with immunofluorescence labeling of CD1 lb and ICAM-1 to study PVs structures. Fibroblasts and leukocytes with positive immunofluorescence labeling of CD1 lb and ICAM-1 were mainly observed in PVs of normal and peritonitis rats. PVs DAPI-stained polymorphic nuclei, and cytomembrane and cytoplasma with positive labeling of CDllb and ICAM-1 increased significantly more in peritonitis rats than in normal rats, indicating that PVs occurrence was closely related to inflammation. Functionally, different current intensities electrical stimulation of abdominal viscera and PVs connected with the abdominal wall had no effect on gastric motility. Acupuncture at point Zhongwan （CV12） inhibited gastric motility in both intact and scissor removal of PVs. Acupuncture at point Zusanli （ST36） increased gastric motility in both intact and scissor removal of PVs. The results show that PVs is not involved in the regulating effect of acupuncture on gastrointestinal motility. Therefore, PVs is obvious in inconformity with the theory of meridian-viscera correlation in clinical acupuncture and moxibustion.