中文摘要：

利用荧光光谱、同步荧光光谱和紫外一可见吸收光谱研究了在PH12．0及不同温度下MnS纳米晶与明胶结合反应的光谱行为。结果表明，在明胶溶液中生成的MnS纳米晶与明胶通过范德华和氢键作用力结合成复合物，使明胶的内源荧光猝灭（属于静态荧光猝灭），而MnS纳米晶却未使明胶色氨酸残基的构象发生变化。根据静态猝灭的Lineweave-Burk方程，计算了温度在293K和305K时MnS纳米晶与明胶的结合常数和热力学参数，得到结合常数分别为4．72×103L·mol-1和3．23×103L·mol-1,焓变（△H）为-23．5kJ·mol-1，熵变（AS）分别为一9．86J·K-1·mol-1和一9．87J·K-1·mol-1，吉布斯自由能变（△G）分别为20．61kJ·mol-1和20．49kJ·mol-1

英文摘要：

Fluorescence spectroscopy, synchronous fluorescence spectroscopy and UV-visible absorp- tion spectroscopy were employed to study the spectral behavior of the binding reaction between MnS nanoerystals and gelatin at different temperatures at pH 12.0. The results showed that the MnS nano- crystals produced in the gelatin solution and gelatin combined to form complexes by van der Waals and hydrogen bond forces, which led to the endogenous fluorescence quenching of the gelatin （a static flu- orescence quenching） while MnS nanocrystals didn＇t cause any conformational changes to the gelatin tryptophan residues. According to the Lineweave-Burk equation for static quenching, the binding con- stant and thermodynamic parameters for MnS nanocrystals binded with gelatin at the temperatures of 293 K and 305 K were calculated, giving the binding constants as 4. 72X10a L 1 mo1-1 and 3.23 X 103 L 1 mol-1 , respectively, the enthalpy change （AH） as --23.5 kJ 1 tool 1, the entropy change （AS） as --9.86 J K 1 , mol 1 and --9.87 J 1 K-1 1 mo1-1 , respectively and the Gibbs free energy changes （AG） as 20.61 kJ 1 mol 1 and 20.49 kJ 1 mol-1 , respectively.