中文摘要：

目的探讨对不同种和地理株旋毛虫进行分子鉴定。方法利用简单重复序列区间-PCR（inter simple sequence repeat-PCR，ISSR-PCR）标准引物对5种旋毛虫及我国旋毛虫7个地理株基因组DNA进行扩增，并观察影响扩增效果的因素。结果5种旋毛虫均分别扩增出了各自的特异性条带：旋毛虫（T1）2条（950bp和850bp），乡土旋毛虫（T2）1条（850bp），布氏旋毛虫（T3）3条（1300bp、950bp和700bp），伪旋毛虫（T4）1条（600bp），纳氏旋毛虫（T7）有4条带（1700bp、1450bp、1050bp、900bp）。我国有6个猪源旋毛虫地理株均扩增出了与T1相同的2个特异性条带（950bp、850bp）。此外，T1、河南株、云南株、哈尔滨株、黑龙江同江株还扩增出了另外2条弱带（500bp和400bp）；但湖北株和天津株则无这2条弱带，湖北株扩增出了1350bp的条带，天津株扩增出了1600bp的条带。非猪源的广西株与T1相比，缺少950bp的条带。对1条旋毛虫肌幼虫，在80％酒精保存24w的肌幼虫，在10％甲醛、0．2％叠氮钠及0．05％柳硫汞溶液保存9w的肌幼虫，应用ISSR-PCR均可扩增出其特异性条带。结论ISSR—PCR用于旋毛虫种的分子鉴定具有良好的稳定性和敏感性；我国6个猪源旋毛虫地理株均为T1，其中云南株、河南株、哈尔滨株及黑龙江同江株可归为一类，湖北株和天津株可归为另一类，来自果子狸的广西株分类地位待定。

英文摘要：

In order to identify different species or isolates of Trichi, mella at molecular level, inter-simple sequence repeat-PCR （ISSR-PCR） was performed to amplify genomic DNA of 5 species of Trichimella and 7 Chinese Trichimella isolates with a standard primer, and to observe the influencing factors of PCR amplification. It was demonstrated that the specific band patterns of 5 species of Trichimella were produced by ISSR-PCR products： T. spiralis （T1） with 2 bands （950bp and 850bp）, T. nativa （T2） with 1 band （850bp） , T. britovi （T3） with 3 bands（1 300bp,950bp and 700bp）, T. pseudospiralis （T4） with 1 band （600bp） , and T. nelsoni （TT） with 4 bands（1 700bp, 1 450bp, 1 050bp and 900bp）. The results of electrophoresis of ISSR-PCR products showed that the 2 specific bands （950bp and 850bp ） of six swine Trichinella isolates from China were the same as T. spiralis （T1）. However, there are additional two faint bands （500bp and 400bp） from T1, Henan, Yun- nan, Haerbin, Heilongjiang Tongjiang isolates, one band （1350bp） from Hubei isolate, and one band （1600bp） from Tianjin i- solate, but the two faint bands of T1 were not amplified from Hubei and Tianjin isolates. One specific band （950bp） was absent in Guangxi isolate comparing with T1. The specific bands of single T. spiralis larva, the larvae conserved in 80 % ethanol for 24 weeks, the larvae stored in 10 % formaldehyde, 0. 2 % sodium azide and 0.05 % merthiotate for 9 weeks were amplified by ISSR-PCR, respectively. In conclusion, ISSR-PCR is sensitive and stable for the molecular identification of Trichinella genus. All of 6 swine isolates from China are identified as T1, the isolates from Yunnan, Henan, Haerbin and Tongjiang in Heilongjian are classified as one group, the isolates from Hubei and Tianjin are classified as another group, the taxonomic position of the isolates from masked civet （Paguma larvata） in Guangxi is not certain.