以红细胞及红细胞膜为材料,研究紫外线引发红细胞及红细胞膜的过氧化反应以及Vc对过氧化反应的影响。用新鲜的猪血制备红细胞和红细胞膜,紫外线照射后,分别用分光光度计和硫代巴比妥酸(TBA)法检测红细胞的溶血度及红细胞、红细胞膜的过氧化程度。结果表明,红细胞的溶血度会随紫外线照射量和放置时间增加,红细胞膜的过氧化程度也会随照射量和浓度增加,但在照射量较小的情况下,红细胞并没有出现脂质过氧化,当照射量大于某一点时,其过氧化程度也会随之增加。在所研究的Vc浓度范围内,Vc浓度越大对红细胞膜的保护效果越明显,而Vc对红细胞的保护作用并不明显。
In order to study the peroxidation reaction of erythrocyte and erythrocyte membrane and their protection with Vc involved in radiation from UV, alternate biological model has been used with erythrocyte and erythrocyte membrane, which is isolated from fresh pig blood. Hemolysis in erythrocyte and peroxidation in erythrocyte and erythrocyte membrane has been measured by the spectral photometer and the thiobarbituric acid (TBA) reaction. With the increment of UV radiation dose and deposited time, the hemolysis in erythrocyte increases, and the peroxidation of erythrocyte membrane also increases when radiation dose is up to some value. However, the peroxidation of erythrocyte hasn't been observed while radiation dose is low. In our study, the bigger concentration of Vc, the more obvious protection to erythrocyte membrane. But the protection of Vc to the erythrocyte is not evidence.