中文摘要：

Angiogenesis 为许多生理、病理学的过程是很重要的。然而， angiogenesis 的分子的机制是不清楚的。阐明 angiogenesis 并且到的分子的机制为 angiogenesis 依赖的疾病开发处理，建立是必要的一在 vitro angiogenesis 合适模型。在这研究，我们基于一台 microfluidic 设备在 vitro angiogenesis 模型创造了一篇小说。我们的模型提供一在里面为 endothelial 房间(EC ) 的象 vivo 一样微型环境文化和监视器到在他们在实时的微型环境的变化的 EC 的反应。为了为研究 angiogenesis， EC 增长上的 pro-angiogenic 因素的效果，迁居和像试管的结构形成评估这台 microfluidic 设备的潜力，被调查。我们的结果证明在 3D 矩阵的 EC 的增长率被 pro-angiogenic 因素显著地支持(随 59.12% 的增加) 。与 pro-angiogenic 因素坡度的刺激，方向性地从低集中移植进 Matrigel 到高集中并且因而的 EC 形成了多房间弦和像试管的结构。这些结果建议设备能为阐明提供一个合适的平台 angiogenesis 并且为为 angiogenesis 依赖的疾病屏蔽 pro-angiogenic 或 anti-angiogenic 药的机制。

英文摘要：

Angiogenesis is very important for many physiological and pathological processes. However, the molecular mechanisms of angiogenesis are unclear. To elucidate the molecular mechanisms of angiogenesis and to develop treatments for ＂angiogenesis-dependent＂ diseases, it is essential to establish a suitable in vitro angiogenesis model. In this study, we created a novel in vitro angiogenesis model based on a microfluidic device. Our model provides an in vivo-like microenvironment for endothelial cells （ECs） cultures and monitors the response of ECs to changes in their microenvironment in real time. To evaluate the potential of this microfluidic device for researching angiogenesis, the effects of pro-angiogenic factors on ECs proliferation, migration and tube-like structure formation were investigated. Our results showed the proliferation rate of ECs in 3D matrix was significantly promoted by the pro-angiogenic factors （with an increase of 59.12%）. With the stimulation of pro-angiogenic factors gradients, ECs directionally migrated into the Matrigel from low concentrations to high concentrations and consequently formed multi-cell chords and tube-like structures. These results suggest that the device can provide a suitable platform for elucidating the mechanisms of angiogenesis and for screening pro-angiogenic or anti-angiogenic drugs for ＂angiogenesis-dependent＂ diseases.