中文摘要：

目的：以类波氏真眼点藻（Eustigmatos cf．polyphem）为材料，建立一种测定微藻细胞内生化组分的便捷方法。方法：利用丌、IR法与Lowry法、重量法及苯酚-硫酸法测定不同氮浓度条件下E.cf.polyphem细胞内蛋白质、脂和碳水化合物含量的时相变化。结果：随时相的迁移，蛋白质不断降低，总脂含量不断升高，碳水化合物先升高后降低；最终总脂含量在低氮组达到最高为66．1％，最高生物量为9．15gL^-1；蛋白质和碳水化合物含量在高氮组达到最高，分别为13．05gL^-1和23．36gL^-1。两种方法测得的结果具有相关性（R^2=0．9820；R^2=0．9531；R^2=0．9033）。结论：FTIR是一种测定微藻内生化组分的高效可靠的方法。

英文摘要：

Objective：To develop a rapid method for determination of biochemical components in microalgae, oleaginous microalga Eustig-matos cf. polyphem was used to study the change rules of intracellular organic macromolecules. Method： The fourier transform infrared spectroscopy （FTIR） and traditional methods were used to measure the contents of biological macromolecules （ protein, lipid, and carbo- hydrate） in E. cf. polyphem cells during the whole cuhure period under different concentrations of nitrogen. Result： With the decrease of protein content, the total lipid content was significantly increased along the growth phase. Meanwhile, carbohydrate content firstly rised and then decreased. Massive lipid accumulation was triggered by low concentration of nitrogen, while the highest total lipid content was 66. 1% obtained in low nitrogen group, with the highest biomass of 9. 15gL^-1 achieved. The highest protein and carbohydrate content obtained in high nitrogen concentration was 13.05 g L^-1 and 23.36 gL^-1, respective, which were higher than that obtained in low nitrogen concentra- tion group. The results obtained with two methods showed a good correlation （ R^2 = 0. 9820 ; R^2 = 0. 9531 ; R^2 = 0. 9033 ）. Conclusion ： The results illustrated that FTIR was an efficient approach for determination of biochemical components in microalgae.