中文摘要：

目的：探讨天麻素（Gastrodin,GAD）对大鼠心脏缺血再灌注损伤的保护作用及其机制。方法：将大鼠随机分为假手术组（Sham组）,缺血/再灌注损伤组（MI/R组）和天麻素低、中、高剂量组（GAD组）。通过结扎冠状动脉左前降支构建心肌缺血再灌注损伤模型。测定各组大鼠乳酸脱氢酶（LDH）、肌酸激酶（CK）、天门冬氨酸氨基转移酶（AST）、心肌梗死面积、心脏收缩和舒张功能、丙二醛（MDA）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、超氧化物歧化酶（SOD）、白细胞介素（IL）-6、IL-1β和肿瘤坏死因子（TNF）-α的表达。结果：天麻素预处理可明显减少心肌梗死面积以及降低LDH、CK、AST、MDA、IL-6、IL-1β和TNF-α表达。此外,天麻素预处理还可改善心脏收缩和舒张功能,增加CAT、GPx和SOD活性。结论：天麻素预处理可通过抑制炎症反应和氧化应激而减轻心脏缺血再灌注损伤。

英文摘要：

Objective：To explore the protective effect and mechanism of Gastrodin in myocardial ischemia reperfusion injury.Method：The rats were randomly divided into Sham group（Sham）,ischemia reperfusion injury group（MI/R）,and Gastrodin（low,middle,high concentration）pretreatment groups（GAD）.The model of MI/R was induced by ligating left anterior descending coronary artery.The myocardial infarct size,the cardial systolic and diastolic function and the expression of LDH,CK,AST,GPX,CAT,SOD,MDA,IL-6,IL-1and TNFwere evaluated.Result：Gastrodin pretreatment significantly decreased myocardial infarct size and attenuated the expression of LDH,CK,AST,MDA,IL-6,IL-1βand TNF-αin the rats suffering from MI/R.Gastrodin upregulated the activity of CAT,GPX and SOD and improved the cardiac systolic and diastolic function in a dose-dependent concentration.Conclusion：Gastrodin pretreatment can reduce the myocardial ischemia reperfusion injury by suppressing inflammation and oxidative stress.