中文摘要：

目的 采用蛋白芯片对慢性粒细胞性白血病（CML）患者白细胞介素（IL）-2、IL-4、IL-12、IL-13、肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）、巨噬细胞炎性蛋白-1α（MIP-1α）7种免疫相关细胞因子进行测定,以评价其细胞因子的表达情况.方法 制作人类细胞因子蛋白质芯片;收集患者及健康人外周血,并提取血清（其中患者25例,对照25例）,用上述芯片及酶联免疫吸附试验（ELISA法）对样本进行测定.结果 CML患者IL-4表达水平为（102±12.33）pg/ml,IL-2表达水平为（78±14.08）pg/ml,均较对照组低（P〈0.05）;CML患者的MIP-1α表达水平与对照组差异无统计学意义.结论 CML患者的细胞免疫与体液免疫均受到一定程度的抑制;MIP-1α在CML发病过程中可能受到p210的抑制.

英文摘要：

Objective To investigate expression of 7 kinds of immune related human cytokines （IL-2, IL-4, IL-12, IL-13, IFN-γ TNF-α, MIP-1α） in CML patients. Methods Pure monoclonal antibody on the prepared NC membrane glass slides under certain environmental condition to make human eytokines protein microarray were spotted and serum samples （25 patients, 25 the normais） were collected. Cytokines concentration in the serum with the protein microarray were tested by ELISA. Results IL-4 and IL-12 serum concentration in CML patients are lower than that of the normal （P 〈0.05）. However. No statistic difference of MIP-1α was found between CML patients the normal. Conclusion Cell mediated immunity and humoral immunity of CML patients are both inhibited in some extent and the expression of MIP-1α may be inhibited by p210 in CML patients.