中文摘要：

背景 O6-methylguanine-DNA-methyltransferase (MGMT ) 是修订从 DNA 把 alkylated 组转移到它的半胱氨酸残余避免 DNA 的反常扭动的酶的特定的 DNA。因此，它是抵抗基因在癌症的治疗指向了的药之一。这研究探索了变成哺乳动物的 cells.Methods 的 MGMT 基因的保护的效果包含 RT-PCR 从人的 hepatocytes 克隆的 MGMT 基因的哺乳动物的表示向量被构造并且经由 liposome 转了进 K562 房间和人的外部血 mononuclear 房间(PBMC ) ，然后，为在 RNA 和蛋白质的基因表示的 assayed 铺平。MTT 试金被用来检查有 MGMT gene.Results MGMT 基因的房间 transfected 的药抵抗成功地被克隆。实时 PCR 证明在在 K562 房间的基因 transfected 组的 mRNA 表示排队， PBMC 是空向量， transfected 组织西方的弄污的 respectively.Results 的 13.4 和 4.0 次在基因 transfected 的 MGMT 的显示出的不同更高的表示比在另外的二个组组织。增加到 7 和 2 的 IC50 值在稳定的 transfected K562 房间和短暂 transfected PBMC.Conclusion 分别地预定原来的值的真核细胞的房间的 alkylating 抵抗在是有产品蛋白质执行保护的函数的 MGMT 基因的 transfected 以后被提高，并且可以在癌症化疗为外部血房间的保护的模型提供引用。

英文摘要：

Background O^6-methylguanine-DNA-methyltransferase （MGMT） is a specific DNA revising enzyme transferring alkylated groups from DNA to its cysteine residue to avoid the abnormal twisting of DNA. Therefore, it is one of the drug resistant genes targeted in the treatment of cancer. This study explored the protective effect of MGMT gene transferred into mammalian cells. Methods Mammalian expression vector containing the MGMT gene cloned from human hepatocytes by RT-PCR was constructed and transferred into K562 cells and human peripheral blood mononuclear cells （PBMCs） via liposome, then assayed for gene expression at RNA and protein levels. MIF assay was used to check the drug resistance of cells transfected with MGMT gene. Results MGMT gene was successfully cloned. Real-time PCR showed that the mRNA expression in gene transfected groups in K562 cell line and PBMC were 13.4 and 4.0 times that of the empty vector transfected groups respectively. Results of Western blotting showed distinct higher expression of MGMT in gene transfected group than in other two groups. The IC50 values increased to 7 and 2 times that of the original values respectively in stable transfected K562 cells and transient transfected PBMC. Conclusion The alkylating resistance of eukaryotic cells is enhanced after being transfected with MGMT gene which protein product performs the protective function, and may provide the reference for the protective model of peripheral blood cells in cancer chemotherapy.