中文摘要：

目的建立简便易行经济适用的乙醇脱氢酶2(ADH2)基因单核苷酸多态性检测方法。方法根据单碱基突变位点的碱基替代情况设计引物,其中一条引物根据突变位点邻近序列设计,人为引入错配碱基,使引物3′端和单碱基突变的一种突变型在PCR扩增后形成一个酶切位点,并应用相应内切酶进行酶切鉴定,通过PCR-RFLP技术判断ADH2基因SNP位点多态性。结果设计一对特异引物并PCR扩增含ADH2多态位点的DNA片段,使用限制性内切酶Bsh1236I酶切判断ADH2位点多态性,PCR和酶切效果均较好。结论应用创造酶切位点PCR-RFLP原理建立的ADH2多态位点检测方法具备简便、经济、快速的特点。

英文摘要：

Objective To develop a appropriate assay for identifying single nucleotide polymorphism (SNP) of alcohol dehydrogenase 2 ADH2) gene. Methods According to base substitution situation of one single base mutational site,we designed the present study primers. One of the primers was designed on the basis of neighbourhood sequence of the mutational site,that is,we made one mismatch base to let product a new enzyme site between the 3 end of the primer and the single base mutation type after the PCR amplification....