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中文摘要：
目的探讨多重聚合酶链反应-限制性片断长度多态性（多重PCR-RFLP）技术在单核苷酸多态性检测中的应用。方法应用多重聚合酶链反应（Multiplex）（多重PCR）原理设计人类X射线交错互补修复基因1（XRCC1基因）194、399位点引物,通过PCR-RFLP技术判断XRCC1基因2个SNP位点多态性。结果设计的两对引物可同时PCR扩增分别含2个多态位点的DNA片段,MspI限制性内切酶酶切可判断2位点多态性,PCR和酶切效果均较好。结论多重PCR-RFLP技术可应用于单核苷酸多态位点的检测,并节省时间和费用,提高检测效率。
英文摘要：
Objective To explore the strategy for identifying single nucleotide polymorphism（SNP）with technique of multi-PCR by primer design combined with PCR-RFLP.Methods Primer was designed with multi-PCR theory,then using PCR-RFLP to identify 2 SNPs of XRCC1 gene.Results One pair of primers could simultaneously amplify DNA segments containing 2 SNP sites by PCR,while MspI restriction enzyme could identify 2 SNPs,both of them showed good effect.Conclusion It was suggested that Multi-PCR combined with PCR-RFLP can be used to identify SNP,and is good in time and money saving and detect efficiency improving.
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