中文摘要：

目的研究附子有效成分次乌头碱和甘草有效成分甘草次酸不同比例配伍对H9c2心肌细胞缺氧缺糖损伤的影响及可能作用机制。方法取长成致密单层的H9c2心肌细胞分成正常组、模型组、地高辛组（80μmol/L）、次乌头碱组（120μmol/L）及1∶0.5组、1∶1组、1∶2组（次乌头碱为120μmol/L,甘草次酸分别为60、120、240μmol/L）,每组6个复孔。正常组加入完全培养基1 ml,模型组加入无糖Earle＇s液1 ml,地高辛组、次乌头碱组和各配伍组分别加相应药时用无糖Earle＇s液稀释成相应浓度,加入1 ml,除正常组外其余各组加药后构建缺氧缺糖模型培养4 h。观察各组H9c2心肌细胞的形态学变化,检测细胞培养上清液中肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）,肌酸肌酶（CK）的含量及Caspase-3、Fas-L、Fas蛋白表达。结果正常组心肌细胞呈贴壁生长,多为梭形,形成不规则的星形,并形成呈放射状排列的细胞簇;模型组心肌细胞胞体折光性下降,胞浆皱缩,可见折光性强的脱壁死亡细胞;各配伍组的细胞形态较模型组均有明显改善,并以配伍组1∶1组最明显。与正常组比较,模型组TNF-α、IL-1β、CK含量及Caspase-3、Fas-L、Fas的蛋白表达均升高（P〈0.01）。与模型组比较,次乌头碱组各组指标均无明显改变（P〉0.05）;而地高辛组和各配伍组不同程度降低TNF-α、IL-1β、CK含量及Caspase-3、Fas-L、Fas蛋白表达,并且以1∶1组效果最好（P〈0.05或P〈0.01）。结论次乌头碱配伍甘草次酸对心肌细胞缺氧缺糖损伤有保护作用,并以1∶1配伍最佳,其作用机制可能与抗炎及抑制细胞凋亡有关。

英文摘要：

Objective To study the effect and possible mechanism of different compatibility proportion of hypaconitine,active constituent of Fuzi（ radix aconiti carmichaeli） and glycyrrhetinic acid active constituent of Gancao（glycyrrhiza uralensis） on H9c2 myocardial cell with oxygen-glucose deprivation. Methods The researchers took H9c2 myocardial cell growing into compact monolayer to divide into a normal group,model group,digoxin group（80μmol /L）,hypaconitine group（ 120 μmol /L）, proportion 1 ∶ 0. 5 group,1 ∶ 1 group and 1 ∶ 2 group,i. e. hypaconitine 120 μmol / L,glycyrrhetinic acid 60,120 and 240 μmol / L respectively,six holes in each group.The researchers put complete medium 1 ml into the normal group and sugar-free Earle＇ s liquid 1 ml into the model group. Put the corresponding medicine into the digoxin group,the hypaconitine group and each compatibility group and used sugar-free Earle＇s liquid to dilute to the corresponding concentration,making the total amount of liquid to1 ml. The other groups after putting into drug were modeled into oxygen-glucose deprivation to cultivate for 4h except the normal group. The researchers observed morphological changes of H9c2 myocardial cell in each group and detected the content of tumor necrosis factor α（ TNF-α）,interleukin 1β（ IL-1β） and creatine kinase（ CK） and the expression of Caspase-3,Fas-L and Fas protein. Results Myocardial cells grew adhering to wall in the normal group and most of them were fusiform with irregular star and formed radiate cell clusters. In the model group,refractivity of myocardial cell body declined with the endochylema shrinking. The researchers found dying cells falling off from wall with strong refractivity. Compared with the model group,cellular form obviously improved in the each compatibility group and the most obviously in the 1 ∶ 1 group. Compared with the normal group,the content of TNF-α,IL-1β and CK and the expression of Caspase-3,Fas-L and Fas protein all increased in the model group（ P?