中文摘要：

目的：研究多聚免疫球蛋白受体（pIgR）在鼻息肉组织中的表达水平,并初步探讨pIgR在鼻息肉发病机制中的作用。方法：选取36例鼻息肉患者的鼻息肉组织标本为实验组,选取同期行鼻内镜治疗的12例鼻中隔偏曲患者为对照组,取下鼻甲组织标本。术后行苏木精-伊红染色评估鼻息肉组织中嗜酸粒细胞（Eos）的浸润情况,并依据Eos的浸润程度分为嗜酸粒细胞性鼻息肉组（Eos CRSwNP组,21例）和非嗜酸粒细胞性鼻息肉组（non-Eos CRSwNP组,15例）。采用免疫组织化学法检测组织中pIgR和IgA的表达情况,实时定量聚合酶链反应（RT-PCR）法检测组织中pIgR、IgA、人类维甲酸孤儿受体（RORc）及叉头转录因子（Foxp3）mRNA的表达水平,统计分析鼻息肉组织中pIgR的表达及与Eos数量、辅助性T细胞（Th17细胞）及调节性T细胞（Treg细胞）转录因子的相关性。结果：pIgR在实验组中的表达显著低于对照组,差异有统计学意义（P〈0.05）;Eos CRSwNP组中pIgR的表达低于non-Eos CRSwNP组,差异有统计学意义（P〈0.05）。IgA在实验组中的表达显著高于对照组,差异有统计学意义（P〈0.05）。RT-PCR分析结果显示：实验组中pIgR mRNA和Foxp3mRNA的表达低于对照组（P〈0.05）,RORc mRNA和IgA mRNA的表达高于对照组（P〈0.05）。实验组中pIgR mRNA与RORc mRNA的表达呈负相关（P〈0.05,r=-0.79）,与Foxp3mRNA的表达无相关性（P〉0.05,r=0.36）。结论：pIgR在鼻息肉组织中的表达下调,可能在鼻息肉的发生和发展中起着重要作用。

英文摘要：

Objective：To explore the expression of polymeric immunoglobulin receptor in nasal polyps of patients with chronic rhinosinusitis with nasal polyps,and to investigateits relationship with the pathogenesis of nasal polyps.Method：Thirty-six specimens of nasal polyps were harvested patients were selected for the control group who had operation of nasal septal construction in the corresponding time period.The pIgR and IgA expression in nasal polyps and normal nasal inferior turbinate mucosa were detected by immunohistochemistry,and the real-time reverse transcription（RT-PCR）were used to detect the level of pIgR,IgA,RORc and Foxp3 mRNA expression in nasal polyps and normal nasal inferior turbinate mucosa.The association between pIgR mRNA and their association with the number of EOS,RORc mRNA,Foxp3 mRNA were analyzed,respectively.Result：The expression of pIgR in the nasal polyps was significantly lowerer than that in control group,and the result was statistically significant（P〈0.05）;Compared with nasal polyps with no eosinophils,the expression levels of pIgR in the nasal polyps with eosinophils was lower、and the result was statistically significant（P〈0.05）.The expression of IgA in the nasal polyps was significantly higherthan that in control,and the result was statistically significant（P〈0.05）.Compared with control,the mRNA expression of pIgR and Foxp3 in the nasal polyps were significantly lower,while the expression levels of IgA mRNA and Foxp3 mRNA in the nasal polyps was significantly higher compared to controls,and the result was statistically significant（P〈0.05）.In nasal polyps,pIgR mRNA expression was correlated with RORc mRNA（P〈0.05,r=-0.79）,and there was no correlation between pIgR mRNA and Foxp3 mRNA（P〈0.05,r=0.36）.Conclusion：It was proved that pIgR down-regulation play an important role in the development of nasal polyps.